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枇杷属植物种间及近缘属杂交亲和性研究
引用本文:李桂芬,杨向晖,乔燕春,高用顺,蒋园园,林顺权.枇杷属植物种间及近缘属杂交亲和性研究[J].园艺学报,2016,43(6):1069-1078.
作者姓名:李桂芬  杨向晖  乔燕春  高用顺  蒋园园  林顺权
作者单位:(1华南农业大学园艺学院,广州 510642;2广西农业科学院园艺研究所,南宁530007)
基金项目:广东省科技基础条件建设领域项目(2015A030303015),广州市科技创新委资助项目(201504010028)
摘    要:采用去雄、套袋、人工授粉方法对7种枇杷属植物及2种枇杷近缘属植物共14份材料配置的91个种间、21个属间共112个杂交组合进行研究,结果发现:91个种间杂交组合中有28个坐果率为零,即杂交不亲和,约占30%;杂交组合大多为部分亲和,仅少数为完全亲和。枇杷属与其近缘属植物(石斑木、石楠)的正交基本不亲和,反交有5个组合能结果,但杂种胚中途败育,需要进行胚挽救。不同的种类作为父本或母本时,坐果率相差较大,以大渡河枇杷,栎叶枇杷、野生普通枇杷和‘解放钟’作母本,平均坐果率高达36.2% ~ 58.2%;而以台湾枇杷及其两个变型和椭圆枇杷作母本,平均坐果率仅为2.9% ~ 16.3%,作父本时,平均坐果率较高,达22.4% ~ 43.1%。花粉管生长的荧光观察显示,台湾枇杷恒春变型作母本与栎叶枇杷杂交为孢子体不亲和。

关 键 词:枇杷  远缘杂交  亲和性  孢子体不亲和  

Study on Interspecific and Intergeneric Hybridization Compatibility of Eriobotrya and Related Genera
LI Gui-fen,YANG Xiang-hui,QIAO Yan-chun,GAO Yong-shun,JIANG Yuan-yuan,LIN Shun-quan,.Study on Interspecific and Intergeneric Hybridization Compatibility of Eriobotrya and Related Genera[J].Acta Horticulturae Sinica,2016,43(6):1069-1078.
Authors:LI Gui-fen    YANG Xiang-hui    QIAO Yan-chun  GAO Yong-shun  JIANG Yuan-yuan  LIN Shun-quan  
Institution:(1College of Horticulture,South China Agricultural University,Guangzhou 510642,China;2Horticulture Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)
Abstract:The cross-compatibility experiments of 91 inter-specific combinations and 21 inter-generic combinations were carried out among 7 Eriobotrya plants and 2 related genera(Raphiolepis indica Lindl. and Photinia serrulata Lindl.)by emasculation,bagging,and artificial pollination. The results showed 28 in 91 inter-specific combinations set no fruit,which means nearly 30% was incompatible. In the rest combinations,most of them were partial cross-compatibility,a few of them were complete cross- compatibility. The cross between Eriobotrya and its related genera plants(R. indica Lindl. and P. serrulata Lindl.)were incompatibility. The back cross of them have 5 compatible combinations could set fruits,which incompatibility developed. The F1 plants could obtain only after embryo rescue. Various species as male or female got difference fruit setting ratio,E. prinoides Rehd. & Wils.,common loquat(E. japonica)and Eriobotrya × daduheensis H. Z. Zhang ex W. B. Liao,Q. Fan et al. as female plant got 36.2%–58.2% average fruit-setting ratio;while E. deflexa Nakai and its two forms,and E. elliptica Lindl. as female plant just got 2.9%–16.3% average fruit-setting ratio,but got as high as 22.4%–43.1% average fruit-setting ratio when they used as male plant. Fluorescent observation of pollen tube growth showed that no fruit-setting from E. deflexa f. koshunensis Nakai × E. prinoides Rehd. & Wils. might be derived from the sporophyte incompatibility.
Keywords:Eriobotrya  distant hybridization  compatibility  sporophyte incompatibility
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