| 蔗糖在延缓青花菜黄化过程中维持其能量和抗氧化力 |
| |
| 引用本文： | 董栓泉,熊茜,王春幸,王鸿飞,邵兴锋,李和生,许凤.蔗糖在延缓青花菜黄化过程中维持其能量和抗氧化力[J].园艺学报,2016,43(9):1825-1833. |
| |
| 作者姓名： | 董栓泉 熊茜 王春幸 王鸿飞 邵兴锋 李和生 许凤 |
| |
| 作者单位： | （宁波大学食品科学与工程系，浙江宁波 315211） |
| |
| 基金项目： | 国家自然科学基金青年科学基金项目(31301574)，浙江省自然科学基金项目(Y16C200011)，浙江省科技厅项目(2014C02023)，宁波市自然科学基金项目(2015A610273)，宁波大学人才引进项目(ZX2012000031)，宁波大学学科项目(xk11344)，宁波大学校科研基金项目(XYL14025) |
| |
| 摘 要： | 为探讨蔗糖处理对青花菜采后黄化的抑制作用,采用含不同浓度(0、50、100、150、200和250 mmol·L~(-1))蔗糖的MS固体培养基培养青花菜花蕾,研究其对青花菜花蕾抗氧化能力与能量代谢的影响。结果发现,含150 mmol·L~(-1)蔗糖的MS培养基处理能显著抑制青花菜叶绿素降解;与空白对照相比,蔗糖处理能显著抑制多酚氧化酶(PPO)活性上升,延缓苯丙氨酸解氨酶(PAL)活性下降,而对抑制丙二醛(MDA)含量上升没有显著效果。蔗糖处理通过提高叶绿体、线粒体中Ca~(2+)-ATPase活性,以及花蕾中三磷酸腺苷(ATP)、二磷酸腺苷(ADP)含量和能荷(EC)水平来维持能量代谢。维生素C含量的下降可能不是叶绿素降解的关键原因。
|
| 关 键 词： | 青花菜 蔗糖 抗氧化酶 能量代谢 |
Sucrose Maintain the Energy and Antioxidant Activity in Delaying the Yellowing of Broccoli |
| |
| DONG Shuan-quan,XIONG Qian,WANG Chun-xing,WANG Hong-fei,SHAO Xing-feng,LI He-sheng,XU Feng.Sucrose Maintain the Energy and Antioxidant Activity in Delaying the Yellowing of Broccoli[J].Acta Horticulturae Sinica,2016,43(9):1825-1833. |
| |
| Authors: | DONG Shuan-quan XIONG Qian WANG Chun-xing WANG Hong-fei SHAO Xing-feng LI He-sheng XU Feng |
| |
| Institution: | （Department of Food Science and Engineering，Ningbo University，Ningbo，Zhejiang 315211，China） |
| |
| Abstract: | The effects of sucrose treatment on antioxidant activity and energy metabolism of broccoli florets were investigated. Broccoli florets were treated with MS solid medium including various concentrations（0，50，100，150，200 and 250 mmol · L-1）of sucrose in dark for 3 d. The results showed the medium with 150 mmol · L-1 of sucrose significantly inhibited the chlorophyll degradation，while the control group turned completely yellow. When compared to the control group，sucrose treatment restrained the increase of polyphenol oxidase（PPO）activity and the decrease of L-phenylalanine ammonia-lyase（PAL）activity. There were no remarkable differences in the increase of malondialdehyde（MDA）content between the sucrose-treated and control groups. Sucrose treatment maintain the energy metabolism by maintaining higher levels of ATP，ADP and energy charge（EC）and delaying the decline of Ca2+-ATPase activity in chloroplast and mitochondria. The declining of the vitamin C may not be the key to chlorophyll degradation. |
| |
| Keywords: | broccoli sucrose antioxidant enzymes energy metabolism |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
