| 山东省芍药红斑病病原菌鉴定 |
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| 引用本文： | 李丽,宋淑香,刘会香,郭先锋.山东省芍药红斑病病原菌鉴定[J].园艺学报,2016,43(2):365-372. |
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| 作者姓名： | 李丽 宋淑香 刘会香 郭先锋 |
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| 作者单位： | 1山东农业大学林学院，山东泰安 271018；2山东农业大学植物保护学院，山东泰安 271018 |
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| 基金项目： | 山东省自然科学基金项目(ZR2014CM028) |
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| 摘 要： | 从山东菏泽和泰安两地感病芍药（Paeonia lactiflora Pall.）植株的叶片上进行了红斑病病原分离纯化和致病力检测，并对致病菌进行了形态学鉴定及rDNA-ITS 序列分析。研究结果表明，病样组织中可分离到两种疑似病原菌，分离比例为4.8︰1，初步鉴定为链格孢（Alternaria alternata）和细极链格孢（A. tenuissima）。进一步rDNA-ITS分析结果表明，两种病原菌5.8S rDNA及其两侧ITS区序列分别与GenBank中序列号为KF380822.1、HM467832.1的同源性最高，均为99%，由此确定两种病原菌分别为链格孢Alternaria alternata和细极链格孢A. tenuissima，其序列已在GenBank登录，登录号分别为KR912224和KR912225。因此，确定山东省芍药红斑病是由链格孢A. alternata和细极链格孢A. tenuissima两种病原菌引起的复合侵染。
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| 关 键 词： | 芍药 红斑病 病原鉴定 链格孢属 rDNA-ITS |
Identification of Red Spot Pathogens on Peony in Shandong Province |
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| LI Li,SONG Shu-xiang,LIU Hui-xiang,GUO Xian-feng,.Identification of Red Spot Pathogens on Peony in Shandong Province[J].Acta Horticulturae Sinica,2016,43(2):365-372. |
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| Authors: | LI Li SONG Shu-xiang LIU Hui-xiang GUO Xian-feng |
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| Institution: | 1.College of Forestry，Shandong Agricultural University，Tai’an，Shandong 271018，China；2College of Plant Protection，Shandong Agricultural University，Tai’an，Shandong 271018，China |
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| Abstract: | Red spot has been one of the most serious diseases in herbaceous peony production. Identification of the pathogen causing the disease is necessary for the determination of effective treatment options. In the present study，firstly，the fungi were isolated and purified from the infected leaves and the pathogenicity was detected by artificial inoculation. Then the morphological characteristics and rDNA-ITS sequence of the pathogens causing red spot disease was studied. The results demonstrated that two kinds of suspected fungi，A and B，were isolated from the disease-infected samples，with the isolation ratio of 4.8︰1. Both fungi were further confirmed pathogenic and therefore determined as the pathogens. The morphological characteristics revealed that these two pathogens were Alternaria alternate and A. tenuissima，which was validated by further rDNA-ITS analysis. ITS1-5.8S rDNA-ITS2 sequences of pathogen A and pathogen B were respectively highly homogenic with those of KF380822.1 and HM467832.1 in GenBank database，hence they were respectively identified as A. alternate and A. tenuissima. The sequences of these two pathogens have been submitted to GenBank，and the accession numbers are KR912224 and KR912225. Based on the above results，it was concluded that red spot diseaseon herbaceous peony in Shandong province was caused by both A. alternate and A. tenuissima. |
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| Keywords: | Paeonia lactiflora red spot pathogen identification Alternaria rDNA-ITS |
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