| 芋淀粉分支酶SBE基因的克隆与表达分析 |
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| 引用本文： | 顾绘,陈赛男,李良俊,程立宝.芋淀粉分支酶SBE基因的克隆与表达分析[J].园艺学报,2016,43(10):2049-2058. |
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| 作者姓名： | 顾绘 陈赛男 李良俊 程立宝 |
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| 作者单位： | （1扬州大学园艺与植物保护学院，江苏扬州 225009；2南通科技职业学院，江苏南通 226007） |
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| 基金项目： | 江苏省科技支撑计划项目(BE2013388) |
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| 摘 要： | 以多子芋品种‘香堂芋’幼嫩叶片为试材，利用RACE结合RT-PCR技术克隆得到全长为3 051 bp的淀粉分支酶SBE基因cDNA序列（登录号：KX013544），其中开放阅读框长2 538 bp，编码845个氨基酸；该序列与玉米SBEII、马铃薯SBEIIa同源性均达85%以上；芋SBE编码蛋白具有N–末端、C–末端和α–淀粉酶催化域。利用RT-PCR技术克隆得到芋SBE的基因组DNA序列，全长为12 362 bp，包括20个外显子和19个内含子。芋球茎形成过程中，子芋植株叶片中SBE表达量显著高于母芋植株叶片、叶柄和根系及母芋等组织，子芋膨大过程中SBE表达量逐渐升高，而孙芋膨大过程中SBE表达量逐渐下降。子芋、孙芋SBE表达对支链淀粉合成积累可能起重要作用。
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| 关 键 词： | 芋 淀粉分支酶基因 克隆 表达 |
Cloning and Expression Analysis of Starch Branching Enzyme Gene in Taro (Colocasia esculenta) |
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| GU Hui,CHEN Sai-nan,LI Liang-jun,CHENG Li-bao.Cloning and Expression Analysis of Starch Branching Enzyme Gene in Taro (Colocasia esculenta)[J].Acta Horticulturae Sinica,2016,43(10):2049-2058. |
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| Authors: | GU Hui CHEN Sai-nan LI Liang-jun CHENG Li-bao |
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| Institution: | （1School of Horticulture and Plant Protection，Yangzhou University，Yangzhou，Jiangsu 225009，China；2Nantong Science and Technology College，Nantong，Jiangsu 226007，China） |
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| Abstract: | In this study，a soluble starch branching gene（SBE）was isolated from the leaf of ‘Xiangtangyu’，a species of taro based on RT-PCR and PCR-RACE methods. The full-length of SBE cDNA（GenBank accession number KX013544）was 3 051 bp in nucleotide containing an open reading frame of 2 538 bp which encoding 845 amino acids. Phylogenetic analysis showed that SBE had more than 85% homolog with maize，potato and rice，respectively. SBE protein contained N-terminal end，C-terminal end and alpha amylase catalytic domain. DNA sequence of SBE was cloned by RT-PCR method，and the full length of DNA was 12 362 bp with 20 exons and 19 introns. During corm formation the expression of SBE in leaf of son-taro plants was significantly higher than that of mother-taro plants，leafstalks，roots，mother-taro and so on，the expression was improved in son-taros，and decreased in grade-taros with development of corm. Totally，we believed that the expression of SBE was synchronous with amylopectin synthesis in son-taros and grade-taros. |
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| Keywords: | Colocasia esculenta starch branching gene clone expression |
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