| 传染性法氏囊病病毒VP5基因的克隆与原核表达 |
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| 引用本文： | 吴琼,马明,何桂梅,陈明勇.传染性法氏囊病病毒VP5基因的克隆与原核表达[J].动物医学进展,2008,29(6). |
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| 作者姓名： | 吴琼 马明 何桂梅 陈明勇 |
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| 作者单位： | 中国农业大学动物医学院,北京,100094 |
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| 摘 要： | 根据GenBank已登录的传染性法氏囊病病毒（IBDV）VP5基因序列,设计合成了一对VP5基因特异性引物,应用RT-PCR技术从IBDV标准毒株中扩增得到VP5基因,并将其克隆到原核表达载体pGEX-6P-1中,构建了重组原核表达质粒pGEX-6P-1-VP5,对重组表达质粒鉴定正确后,转化大肠埃希菌BL21进行诱导表达,SDS-PAGE和Western blot检测结果表明,IBDV VP5基因在大肠埃希菌BL21中得到了正确表达,所表达的融合蛋白与IBDV阳性血清具有特异性抗原抗体反应。
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| 关 键 词： | 传染性法氏囊病病毒 VP5基因 基因克隆 表达 |
Cloning and Prokaryotic Expression of VP5 Gene of Infectious bursal disease virus |
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| WU Qiong,MA Ming,HE Gui-mei,CHEN Ming-yong.Cloning and Prokaryotic Expression of VP5 Gene of Infectious bursal disease virus[J].Progress In Veterinary Medicine,2008,29(6). |
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| Authors: | WU Qiong MA Ming HE Gui-mei CHEN Ming-yong |
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| Abstract: | According to the published sequence of VP5 gene of IBDV, a pair of specific primers were designed and synthesized. The VP5 gene was amplified by RT-PCR method from IBDV, and cloned into pGEX-6P-1 vector. The prokaryotic expression plasmid containing VP5 gene was successfully constructed. The expression of recombinant plasmid pGEX-6P-1-VP5 in E.coli BL21 was induced and detected by SDS-PAGE and Western-blot analysis. The results showed that the VP5 gene of IBDV can express successfully in E.coli BL21.The GST-VP5 fusion protein which was expressed in E.coli BL21 can be recognized by the positive serum of IBDV. |
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| Keywords: | Infectious bursal disease virus VP5 gene gene cloning expression |
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