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| 剪秋罗种子催芽及秋水仙碱诱导多倍体研究 | |
| 引用本文： | 肖宁,李旦,武永明,曹芹,夏昶,张金凤.剪秋罗种子催芽及秋水仙碱诱导多倍体研究[J].中国农学通报,2012,28(16):208-214. |
| 作者姓名： | 肖宁 李旦 武永明 曹芹 夏昶 张金凤 |
| 作者单位： | 1. 林木育种国家工程实验室/林木、花卉遗传育种教育部重点实验室/国家林业局树木花卉育种与生物工程重点开放实验室/北京林业大学,北京,100083 2. 山西省娄烦县云顶山自然保护区,山西娄烦,030300 3. 山西省繁峙县砂河镇五台山林业局,山西繁峙,034302 4. 河北省隆化县林业局,河北隆化,068150 |
| 基金项目： | 国家林业局林业公益性行业专项“重要乡土树种核心种质评价及高效育种共性技术研究”（201004009）; 北京市园林绿化局花卉产业处课题“草花多倍体新品种培育”（YLHH201100111）; 中央高校基本科研业务费专项资金“油松樟子松种间杂交及扦插繁殖材料幼化技术研究”（JD2011-4） |
| 摘 要： | 为了解决剪秋罗种子发芽率不高的问题,以剪秋罗种子为试验材料,比较种子新旧、播种基质以及不同浓度赤霉素(GA3)对剪秋罗种子发芽势和发芽率的影响,并在此基础上利用不同浓度秋水仙碱和不同时间处理剪秋罗萌动种子诱导多倍体。通过形态鉴定、气孔鉴定以及细胞学鉴定变异植株。结果表明:存放1年的剪秋罗种子发芽指标均优于当年新种子,当年种子可能具有初生休眠特性。以150mg/L的GA3预处理存放1年的剪秋罗种子24h并播种于培养皿中能获得最高的发芽势(76.33%)和最高的发芽率(85.33%)。经过秋水仙碱处理的种子普遍存活率低,但每个处理浓度下都获得了变异植株,秋水仙碱浓度0.1%为筛选出的最适诱导浓度。变异植株表现出了与对照植株在形态上、气孔分析上和细胞学检测上的差异。经鉴定,初选出1株多倍体。研究为培育剪秋罗多倍体新品种探索出了一条新的育种途径。 |
| 关 键 词： | 剪秋罗 发芽率 萌动种子 秋水仙碱 多倍体 |
| 收稿时间： | 2011/12/20 0:00:00 |
| 修稿时间： | 2012/2/11 0:00:00 |
Seeds Germination and Polyploid Induction by the Colchicine of Lychnis fulgens Fisch. |
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| Xiao Ning , Li Dan , Wu Yongming , Cao Qin , Xia Chang , Zhang Jinfeng.Seeds Germination and Polyploid Induction by the Colchicine of Lychnis fulgens Fisch.[J].Chinese Agricultural Science Bulletin,2012,28(16):208-214. | |
| Authors: | Xiao Ning Li Dan Wu Yongming Cao Qin Xia Chang Zhang Jinfeng |
| Institution: | 1National Engineering Laboratory for Tree Breeding/ Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education/ The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration/ Beijing Forestry University, Beijing 100083; 2Genting Mountain Nature Reserve, Loufan Shanxi 030300; 3Wutai Mountain Forestry Administration, Fanshi Shanxi 034302;4Longhua Forestry Administration, Longhua Hebei 068150) |
| Abstract: | In order to solve the problem of low germination rate in Lychnis fulgens seeds, influence on germinative force and germination rate in seed of Lychnis fulgens between stored and new seeds, different planting substrate and different concentrations of gibberellic acid （GA3） pretreatment were compared. The result showed one year of stored seeds were better than when new seeds on seed indicators, then the seed may have a newborn sleep characteristics. Using 150 mg/L of GA3 pretreated the stored seeds for 24 h sown in petri dishes could get the highest germinative force of 76.33% and the highest germination rate of 85.33% . Germinated seeds were treated by different concentration of colchicine （0.05%, 0.10%, 0.20%） and different treating time （12 h, 24 h, 36 h, 48 h） to induce polyploidy. And morphologic, stoma and cytologic comparison between treated plants and CK were studied. The survival rate was generally low after colchicine treatment. Variative plants were obtained under each colchicine concentration and concentration of 0.10% was the optimal induction concentration with the higher mutagenic rate and lower death rate. Varieties plants showed differences on morphologic, stoma and cytological analysis to control plants. One polyploidy was obtained after identification. This study opened up a new way for breeding new cultivar of Lychnis fulgens. |
| Keywords: | Lychnis fulgens germination rate germinated seed colchicine polyploidy |
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