| 不同方法制备鸡胚成纤维细胞的比较试验 |
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| 引用本文： | 牛家强,支海兵,徐业芬,陈国先,吴华伟.不同方法制备鸡胚成纤维细胞的比较试验[J].中国家禽,2003,25(5):11-12. |
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| 作者姓名： | 牛家强 支海兵 徐业芬 陈国先 吴华伟 |
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| 作者单位： | 1. 西藏农牧学院牧医系,西藏,林芝,860000
2. 中国兽医药品监察所,北京,海淀,100081 |
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| 摘 要： | 采用传统法、半胚法和全胚法制备鸡胚成纤维细胞(CEF)单层。结果表明:在同等条件下,均以成纤维细胞为主,并伴有少量的上皮细胞,仅有全胚法含有极少量的肝细胞;培养时细胞贴壁和生长状况无明显差异,其产量稍有差异(全胚法>半胚法>传统法)。
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| 关 键 词： | 上皮细胞 肝细胞 产量 制备方法 传统法 全胚法 半胫法 鸡胚成纤维细胞 |
Comparison of different methods producing chicken embryo fibroblast |
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| Niu Jiaqiang ,Zhi Haibin ,Xu Yefen ,Chen Guoxian ,Wu Huawei.Comparison of different methods producing chicken embryo fibroblast[J].China Poultry,2003,25(5):11-12. |
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| Authors: | Niu Jiaqiang Zhi Haibin Xu Yefen Chen Guoxian Wu Huawei |
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| Institution: | Niu Jiaqiang 1,Zhi Haibin 2,Xu Yefen 1,Chen Guoxian 2,Wu Huawei 2 |
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| Abstract: | Three methods producing fibroblast of chicken embryo,including classic,non -whole embryo and whole embryo methods,were compared in the experiment.Under the same condition,the cells produced by these methods were mostly fibroblasts,accompanied with epithelium,and a particle of liver cells only by whole embryo method.There was no significant difference between the growth situation and attachment of fibroblast to the container wall when cultured and was a bit of difference in production between different methods. |
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| Keywords: | fibroblast epithelium liver cell quantity of production |
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