| Cloning and Identification of S Gene from Chinese Isolate TH—98 of Transmissible Gastroenteritis Virus |
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| 引用本文： | RENXiao－feng LIYI－jing 等.Cloning and Identification of S Gene from Chinese Isolate TH—98 of Transmissible Gastroenteritis Virus[J].东北农业大学学报(英文版),2002,9(1):49-54. |
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| 作者姓名： | RENXiao－feng LIYI－jing |
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| 作者单位： | VeterinaryMedicineCollege,College,NortheastAgriculturalUniversity,Harbin,150030,PRC |
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| 摘 要： | Chinese isolate of transmissible gastroenteritis virus(TGEV) was propagated and harvested in swine testicle(ST) cells.Two pairs of primers were designed according to the published sequence with Oligo 4.1 and DNasis softwares.The products of RT-PCR were named Sa and Sb,of 2.3kb and 2.1kb respectively.Sa was inserted in EcoR Iand Kpn I sites after Sb was cloned in Kpm I and Pst I sites of the same pUC18 plasmid,The recombinant designated pUC-S was verified and analyzed by corresponding restriction endonuclease (RE) and nested PCR on the basis of genetic sites of S gene and physical map of pUC18 plasmid,which was identified as S gene from CHinese isolate of TGEV.
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| 关 键 词： | 传染性胃肠炎病毒 中国分离株TH－98 猪病毒 S基因 基因克隆 鉴定 |
Cloning and Identification of S Gene from Chinese Isolate TH-98 of Transmissible Gastroenteritis Virus |
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| REN Xiao-feng,LI Yi-jing,LI Guang-xing,WANG Jun-wei,LIU Bao-quan.Cloning and Identification of S Gene from Chinese Isolate TH-98 of Transmissible Gastroenteritis Virus[J].Journal of Northeast Agricultural University,2002,9(1):49-54. |
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| Authors: | REN Xiao-feng LI Yi-jing LI Guang-xing WANG Jun-wei LIU Bao-quan |
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| Abstract: | Chinese isolate of transmissible gastroenteritis virus(TGEV)was propagated and harvested in swine testicle(ST)cells.Two pairs of primers were designed according to the published sequence with Oligo 4.1 and DNasis softwares.The products of RT-PCR were named Sa and Sb,of 2.3kb and 2.1kb respectively.Sa was inserted in EcoR I and Kpn I sites after Sb was cloned in Kpn I and Pst I sites of the same pUC18 plasmid.The recombinant designated pUC-S was verified and analyzed by corresponding restriction endonuclease(RE)and nested PCR on the basis of genetic sites of S gene and physical map of pUC18 plasmid,which was identified as S gene from Chinese isolate of TGEV. |
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| Keywords: | transmissible gastroenteritis virus S gene cloning |
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