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| 甘薯茎尖培养与脱毒技术研究 | |
| 引用本文： | 何新民,蒋菁,唐洲萍,唐家文,刘颂东,刘义明.甘薯茎尖培养与脱毒技术研究[J].广西农业科学,2009,40(8):964-968. |
| 作者姓名： | 何新民 蒋菁 唐洲萍 唐家文 刘颂东 刘义明 |
| 作者单位： | 1. 广西农科院经济作物研究所,南宁,530007 2. 东兴市农业局,广西,东兴,538100 3. 防城港市农业局,广西防城港,538001 |
| 摘 要： | 以红姑娘2号甘薯为材料，研究6-BA、NAA、GA，不同配组对茎尖培养的影响，并采用温水钝化法及适宜的茎尖大小来提高红薯病毒脱毒率，用指示植物法对再生植株无性系进行病毒检测。研究结果表明，培养基中的6-BA、NAA、GA，不同浓度配比对红姑娘2号甘薯茎尖培养不定芽、不定根的诱导有明显的影响。在诱导产生愈伤组织和丛芽阶段，培养基MS＋6-BA1．0mg／L＋NAA0．01mg／L＋GA30．1mg／L效果较好。而1／2MS＋NAA0．2mg／L是红姑娘2号试管苗的适宜生根培养基。通过指示植物法检测90个无性系，平均脱毒率为96．7％。 |
| 关 键 词： | 甘薯 茎尖培养 脱毒 病毒检测 |
Development of technology for production of virus free plantlets of sweet potato using the shoot tip culture |
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| HE Xin-min,JIANG Jing,TANG Zhou-ping,TANG Jia-wen,LIU Song-dong,LIU Yi-ming.Development of technology for production of virus free plantlets of sweet potato using the shoot tip culture[J].Guangxi Agricultural Sciences,2009,40(8):964-968. | |
| Authors: | HE Xin-min JIANG Jing TANG Zhou-ping TANG Jia-wen LIU Song-dong LIU Yi-ming |
| Institution: | HE Xin-min, JIANG Jing, TANG Zhou-ping, TANG Jia-wen, LIU Song-dong, LIU Yi-ming(1 Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2 Agricultural Bureau of Dongxing City, Dongxing, Guangxi 538100, China; 3 Agricultural Bureau of Fangchenggang City, Fangchenggang, Guangxi 538001, China) |
| Abstract: | The present study has been carried out to develop virus free plantlets of sweet potato using the shoot tip culture. The sweet potato variety Hongguniang 2 was used as experimental materials to study effects of various concentrations and combinations of 6-BA, NAA and GA3, size of the shoot tip and soaking at different temperature for different times on virus free shoot tip culture, The virus in regenerating plantlet clones was detected using indicator plant method. The results showed that different combinations with different concentrations of 6-BA, NAA, GA3 in medium had significant effect on the induction of adventitious buds and roots in variety Hongguniang 2. The best medium for induction of callus and cluster buds was found to be MS ＋ 1.0 mg/L 6-BA＋ 0.01 mg/L NAA ＋ 0.1 mg/L GA3. The optimum medium for the rooting was 1/2MS ＋ 0.2 mg/L NAA. Further, the average virus-free plantlet production rate was found to be 96.7% for the tested 90 plantlet clones. |
| Keywords: | sweet potato shoot tip culture virus-free detection of virus |
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