Lipophilic hydroxytyrosyl esters. Antioxidant activity in lipid matrices and biological systems |
| |
| Authors: | Trujillo Mariana Mateos Raquel Collantes de Teran Laura Espartero José L Cert Rosa Jover Maria Alcudia Felipe Bautista Juan Cert Arturo Parrado Juan |
| |
| Institution: | Departamento de Química Organica y Farmacéutica and Departamento de Bioquímica, Facultad de Farmacia, Universidad de Sevilla, C/Prof. García Gonzalez 2, 41012 Sevilla, Spain. |
| |
| Abstract: | Antioxidant activities of lipophilic hydroxytyrosyl acetate, palmitate, oleate, and linoleate were compared with those of hydroxytyrosol, alpha-tocopherol, and butylhydroxytoluene (BHT) in both glyceridic matrix and biological systems. Aliquots of a glyceridic matrix spiked with various concentrations of antioxidant were subjected to accelerated oxidation in a Rancimat apparatus operated at 90 degrees C. The relationships between induction time (IT) and antioxidant concentration (mmol/kg) presented by hydroxytyrosol and hydroxytyrosyl acetate, palmitate, oleate, and linoleate were similar. Hydroxytyrosol and its esters showed greater antioxidant activity than alpha-tocopherol or BHT. We also evaluated the capacity of hydroxytyrosyl esters to protect proteins and lipids against oxidation caused by peroxyl radicals, using a brain homogenate as an ex vivo model. All tested compounds showed a protective effect in these systems, which was greater in preventing the generation of carbonyl groups in protein than of malondialdehyde in lipid. Inclusion of a lipophilic chain in the hydroxytyrosol molecule enhanced its antioxidant capacities in this biological model. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
