| 转基因与常规杂交相结合改良水稻耐盐性 |
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| 引用本文： | 郭龙彪,薛大伟,王慧中,陈受宜,卢德赵,曾大力,高振宇,颜美仙,黄大年,钱前.转基因与常规杂交相结合改良水稻耐盐性[J].中国水稻科学,2006,20(2):141-146. |
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| 作者姓名： | 郭龙彪 薛大伟 王慧中 陈受宜 卢德赵 曾大力 高振宇 颜美仙 黄大年 钱前 |
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| 作者单位： | 1.中国水稻研究所 水稻生物学国家重点实验室， 浙江 杭州 310006； 2中国科学院 遗传与发育生物学研究所， 北京 100101； 3浙江大学 生命科学学院， 浙江 杭州 310029; #共同第一作者 |
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| 基金项目： | 高比容电子铝箔的研究开发与应用项目;中国科学院资助项目 |
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| 摘 要： | 通过农杆菌介导法和基因枪法将CMO、BADH、mtlD、gutD和SAMDC基因以单价或双价的形式导入水稻常规品种中，再结合常规杂交育种，选育5价强耐盐性的转基因水稻植株。1~5价9类组合的水稻品系，经PCR分子检测，在转基因后代中多价目的基因聚合，遗传稳定，且分子检测与田间耐盐性的表现一致。转基因植株在盐碱地中能正常生长，拓展了水稻常规品种耐盐性。并已获得耐0.5%~1.0%NaCl的T秀水11——品3、品6和品7等9份优良株系或中间材料。
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| 关 键 词： | 耐盐相关基因 遗传转化 常规杂交 新种质 |
| 文章编号： | 1001-7216（2006）02-0141-06 |
| 收稿时间： | 2005-01-08 |
| 修稿时间： | 2005-09-27 |
Improvement of Rice Salt-Tolerance by Using an Integrated Method of Gene Transformation and Traditional Breeding |
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| GUO Long-biao,XUE Da-wei,WANG Hui-zhong,CHEN Shou-yi,LU De-zhao,ZENG Da-li,GAO Zhen-yu,YAN Mei-xian,HUANG Da-nian,QIAN Qian.Improvement of Rice Salt-Tolerance by Using an Integrated Method of Gene Transformation and Traditional Breeding[J].Chinese Journal of Rice Science,2006,20(2):141-146. |
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| Authors: | GUO Long-biao XUE Da-wei WANG Hui-zhong CHEN Shou-yi LU De-zhao ZENG Da-li GAO Zhen-yu YAN Mei-xian HUANG Da-nian QIAN Qian |
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| Institution: | 1 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China ;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101 , China;3. College of Life Science, Zhejiang University , Hangzhou 310029, China ; These authors contributed equally to this paper |
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| Abstract: | The genes encoding enzymes responsible for synthesizing some low-molecular weight metabolites, such as betaine, polyamines, and mannitol,were transformed into rice plants to enhance the salt- tolerance. The improvement of rice salt- tolerance with five target genes (BADH, CMO, mtlD, gutD and SAMDC) was conducted through gene transformation and traditional breeding approaches. Results indicated that the genes were successfully transformed into rice by particle bombardment or mediated by Agrobacterium tumefaciens and integrated into the genome of the transgenic plants by using PCR detections. The salt tolerance of the transgenic plants and their progenies with 1-5 genes was significantly enhanced and stably maintained in the salt-stress fields. Nine elite rice transgenic lines such as Pin 3, Pin 6 and Pin 7 from Xiushui 11 were obtained, which grew normally in the salt pool containing 0.5%-1.0% NaCl. |
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| Keywords: | genes related to salt-tolerance transformation traditional breeding elite germplasm rice (Oryza sativa) |
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