| 红海榄液泡膜Na+/H+逆向转运蛋白基因的克隆与分析 |
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| 引用本文： | 翟金玲,徐远峰,马宏伟,陈旭峰,黄惜.红海榄液泡膜Na+/H+逆向转运蛋白基因的克隆与分析[J].热带生物学报,2011(1):6-13,20. |
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| 作者姓名： | 翟金玲 徐远峰 马宏伟 陈旭峰 黄惜 |
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| 作者单位： | 热带生物资源教育部重点实验室；海南大学农学院,海南海口570228 |
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| 基金项目： | 人力资源与社会保障部2008年度高层次留学人才回国工作资助项目; 海南大学2009年度科研项目（hd09xm60）; 海南省自然科学基金项目（808109）资助 |
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| 摘 要： | 根据液泡膜Na+/H+反转运蛋白基因(fHXl)的保守序列设计引物,通过RT-PCR扩增得到红海榄Na+/H+反转运蛋白基因的cDNA片段,然后采用cDNA末端快速扩增法(RACE)得到RsHX1基因的全长cDNA序列,GenBank登录号为FJ627273,被命名为RsNHXl.该基因序列的长度为2 094 bp,包...
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| 关 键 词： | 红海榄 耐盐 Na+/H+反转运蛋白基因(NHX1) |
Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na^＋/H^＋ Antiporter of Rhizophora stylosa（RsNHX1） |
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| ZHAI Jin-ling,XU Yuan-feng,MA Hong-wei,CHEN Xu-feng,HUANG Xi.Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na^＋/H^＋ Antiporter of Rhizophora stylosa（RsNHX1）[J].Journal of Tropical Biology,2011(1):6-13,20. |
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| Authors: | ZHAI Jin-ling XU Yuan-feng MA Hong-wei CHEN Xu-feng HUANG Xi |
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| Institution: | (Key Laboratory of Tropic Biological Resources of Ministry of Education/Institute of Bio Science and Technology,Hainan University,Haikou 570228,China) |
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| Abstract: | Based on consensus sequence of vacuolar-type Na^＋/H^＋ antiporter genes（NHX1）,the primers were designed to isolate a cDNA fragment of NHX1 from Rhizophora stylosa by RT-PCR.The full length cDNA was obtained by rapid amplification of cDNA ends（RACE）,and the gene was termed as RsNHX1.This gene is 2 094 bp in length and contains one open reading frame of 1 614 bp,encoding 538 amino acid residues.Accession number of GenBank is FJ627273.Bioinformatics analysis results showed that the amino acid sequence of the RsNHX1 gene is 74% and 69% similar with that of PeNHX1（ABY86892,Populus euphratica） and AtNHX1（AAM34759,Arabidopsis thaliana）,respectively.There are significant differences among these sequences. |
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| Keywords: | Rhizophora stylosa salt-tolerance Na^＋/H^＋ antiporter genes（NHX1） |
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