| 合浦珠母贝抗菌肽粗提液制备方法的优化 |
| |
| 引用本文： | 于文媛,王世锋,谢珍玉,陈诏,周永灿.合浦珠母贝抗菌肽粗提液制备方法的优化[J].热带生物学报,2011(1):14-20. |
| |
| 作者姓名： | 于文媛 王世锋 谢珍玉 陈诏 周永灿 |
| |
| 作者单位： | 海南大学海洋学院／海南省热带水生生物技术重点实验室,海南海口570228 |
| |
| 基金项目： | 国家自然科学基金项目（40866002）; 农业科技成果转化项目（2009GB2E200302）; 人事部留学人员科技活动项目; 海南省自然科学基金项目（808117）; 海南大学2009年度科研项目（hd09xm57） |
| |
| 摘 要： | 研究了不同处理方法制备合浦珠母贝外套膜和鳃组织抗菌肽粗提液的抗菌活性,结果表明：以1.0×106cfu·mL^-1无乳链球菌刺激合浦珠母贝14 h以内对其粗提液抗菌活性无显著影响;合浦珠母贝外套膜或鳃组织抗菌肽粗提液制备时,IKAT-18匀浆机的匀浆效果要优于组织匀浆器和研钵。100℃水浴处理组织粗提液10 m in可显著提高其对溶藻弧菌的抗菌活性;反复冻融则会显著降低外套膜组织粗提液的抗菌效果。
|
| 关 键 词： | 合浦珠母贝 抗菌肽 分离方法 优化 |
Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii |
| |
| YU Wen-yuan,WANG Shifeng,XIE Zhen-yu,CHEN-zhao,ZHOU Yong-can.Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii[J].Journal of Tropical Biology,2011(1):14-20. |
| |
| Authors: | YU Wen-yuan WANG Shifeng XIE Zhen-yu CHEN-zhao ZHOU Yong-can |
| |
| Institution: | (Key Laboratory of Tropical Aquatic Biotechnology of Hainan Province;College of Marine Sciences,Hainan University,Haikou 570228,China) |
| |
| Abstract: | The different processing methods were performed to extract the crude antimicrobial peptides extraction from the mantle and gill tissues of Pincatda martensii,and the activities of crude extractions were compared.The result suggested that the activities of crude extractions were not changed when Pincatda martensii was induced by Streptococcus agalactiae with 1.0×106 cfu·mL-1 for 14 h;the antibacterial activity of crude extractions homogenated by IKAT-18 homogenate machine was higher than that by the tissue grinder and the agate mortar;the antibacterial activity against Vibrio alginolyticus was significantly improved when the extraction was heated at 100 ℃ for 10 min;and the antibacterial activity against V.alginolyticus was significantly reduced when the crude extractions of the mantle tissue from P.martensii was frozen and thawed repeatedly. |
| |
| Keywords: | Pincatda martensii antimicrobial peptides isolation optimization |
| 本文献已被 维普 等数据库收录！ |
