| 黄鳝皮肤白蛋白的纯化及性质 |
| |
| 引用本文： | 张英霞,韦双双,陆露,陈聪伟,迟婷婷,安天琛,关怀,满初日嘎.黄鳝皮肤白蛋白的纯化及性质[J].热带生物学报,2011(3):226-229. |
| |
| 作者姓名： | 张英霞 韦双双 陆露 陈聪伟 迟婷婷 安天琛 关怀 满初日嘎 |
| |
| 作者单位： | [1]海南大学热带生物资源教育部重点实验室／海洋生物教学示范中心,海南海口570228 [2]海口VTI生物研究院,海南海口570228 |
| |
| 基金项目： | 国家自然科学基金项目（30700128） |
| |
| 摘 要： | 通过阴离子交换层析和凝胶过滤层析,从黄鳝(Monopterus albus)皮肤中得到其白蛋白,命名为MAA-skin.该蛋白为单链蛋白质,相对分子质量为67 ×103,经Schiff's试剂鉴定为非糖蛋白,其N-末端氨基酸序列为GHVKW.用纯化的黄鳝血清白蛋白(MAA -serum)为免疫原免疫大白兔,制备抗MAA...
|
| 关 键 词： | 黄鳝 血清白蛋白 分离纯化 |
Purification and Characteristic of Albumin from Monopterus albus Skin |
| |
| Institution: | ZHANG Ying-xia, WEI Shuang-shuang, LU Lu, CHEN Cong-wei, CHI Ting-ting, AN Tian-chen, GUAN Huai, MANCHU Ri-ga1 ( 1. Key Laboratory of Tropic Biological Resources, Minister of Education, Hainan University, Haikou 570228, China; 2. Haikou VTI Biological Institute, Haikou 570228, China) |
| |
| Abstract: | The anion exchange chromatography and gel filtration chromatography were performed and skin albu- min of Monopterus albus was obtained. The protein is a single chain protein with molecular weigh of 67 ~ 103, and named MAA-skin. MAA-skin was determined as a glycoprotein by Sciff~ agent. The N-terminal amino acid sequence is GHVKW. The purified MAA-serum was used to immunize rabbits to prepare polyclonal antibody. ELISA data demonstrated that the antibody titer of the MAA-skin was up to 1 ： 10 000. Western blot results indi- cated that the polyclonal antibodies raised against MAA-serum strongly cross-reacted with both MAA-serum and MAA-skin with a compatible grade. The distribution of frog albumin in frog skin suggested that it plays important roles in skin physiological functions, such as water salt metabolism, osmotic pressure maintaining and substance transporting, etc. |
| |
| Keywords: | Monopterus albus albumin skin |
| 本文献已被 维普 等数据库收录！ |
|
