| DNA提取方法对转基因甘蔗PCR检测的影响 |
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| 引用本文： | 王晓音,赵婷婷,冯翠莲,张树珍.DNA提取方法对转基因甘蔗PCR检测的影响[J].热带生物学报,2011(2):138-142. |
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| 作者姓名： | 王晓音 赵婷婷 冯翠莲 张树珍 |
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| 作者单位： | [1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 |
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| 基金项目： | 基金项目：中央级公益性科研院所基本科研业务费（ITBBZD0724） |
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| 摘 要： | 研究了不同提取方法和不同生长时期甘蔗提取的DNA对转基因甘蔗植株PCR检测的影响。结果表明,幼苗时期提取的甘蔗叶片模板DNA质量高于田间成熟植株;甘蔗幼苗时期的PCR检测易于成熟植株;CTAB法、SDS法和CTAB-SDS法提取的甘蔗叶片DNA均能满足幼苗期甘蔗抗性植株的PCR检测要求;对成熟转基因甘蔗植株而言,以CT...
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| 关 键 词： | 转基因甘蔗 DNA提取 PCR检测 |
Effects of DNA Extraction Methods on Transgenic Sugarcane PCR Detection |
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| WANG Xiao-yin,ZHAO Ting-ting,FENG Cui-lian,ZHANG Shu-zhen.Effects of DNA Extraction Methods on Transgenic Sugarcane PCR Detection[J].Journal of Tropical Biology,2011(2):138-142. |
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| Authors: | WANG Xiao-yin ZHAO Ting-ting FENG Cui-lian ZHANG Shu-zhen |
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| Institution: | 1. College of Agriculture, Hainan University, Haikou 570228, China; 2. Institute of Tropical Bioscience and Biotechnology, CATAS/Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops, Haikou 571101, China) |
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| Abstract: | The effects of three template DNA extraction methods from different growth stages on transgenic sugarcane PCR detection were compared. The results indicated that the quality of DNA extracting from seedling leaves was better than that of mature plants and mature stage, and PCR detections were easier; DNA extracted from sugarcane leaves with CTAB method, SDS method and CTAB-SDS method were all suitable for PCR detection of resistant plants in seedling stage. The quality of DNA extracted with CTAB-SDS method was the best for PCR detection of resistant plants in mature stage. |
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| Keywords: | transgenic sugarcane DNA extraction PCR |
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