Malathion carboxylesterase enzymes in Anopheles arabiensis from Sudan |
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| Authors: | Janet Hemingway |
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| Institution: | Department of Entomology, London School of Hygiene and Tropical Medicine, Keppel St (Gower St.), London WC1E 7HT, England |
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| Abstract: | Malathion resistance in Anopheles arabiensis from Sudan is monofactorially inherited and is expressed in the adults but not in the larvae. The resistance is suppressed by the esterase synergist, triphenylphosphate. Semipurification of the soluble esterase enzymes by Sephadex G-25 and Sephacryl S-200 gel filtration revealed no difference between the enzymes of the resistant and susceptible strains with α- or β-naphthylacetate (NA) with a fixed substrate concentration in either the adults or larvae. However, with the malathion-specific assay a second peak of activity was observed in the adult resistant strain which was not present in either the larvae of this strain or the larvae and adults of the susceptible strain. A corresponding threefold difference in the Km value for α-NA was also observed in the resistant adults over the range of this second peak, but there was no change in the Km with β-NA. |
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