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一种引起蛋鸡产蛋下降的新型黄病毒的分离与初步鉴定
引用本文:陈仕龙,陈少莺,王劭,林锋强,江斌,程晓霞,黄梅清,朱小丽,李兆龙.一种引起蛋鸡产蛋下降的新型黄病毒的分离与初步鉴定[J].福建农业学报,2011,26(2):170-174.
作者姓名:陈仕龙  陈少莺  王劭  林锋强  江斌  程晓霞  黄梅清  朱小丽  李兆龙
作者单位:福建省农业科学院畜牧兽医研究所;福建省畜禽疫病防治工程技术研究中心;
基金项目:福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y02)
摘    要:从蛋鸡临床表现为产蛋下降、卵泡萎缩、卵泡膜出血为主要特征的发病蛋鸡的卵巢、输卵管、肝、脾等组织中分离到3株病毒.分离毒能致死番鸭胚和鸡胚,人工感染产蛋期的蛋鸡能导致蛋鸡产蛋急速下降,卵泡出血和萎缩,并能从人工感染病鸡中回收到病毒;分离毒不能凝集鸡和鸽红细胞,并对乙醚、氯仿敏感;病毒核酸为RNA;PCR或IFA结果排除了...

关 键 词:蛋鸡  产蛋下降  新型鸡黄病毒  分离与鉴定

Isolation and Identification of New Flaviviruses Causing Reduction on Egg-laying in Chicken
CHEN Shi-long,CHEN Shao-ying,WANG Shao,LIN Feng-qing,JIANG Bin,CHENG Xiao-xia,HUANG Mei-qing,ZHU Xiao-li,LI Zhao-long.Isolation and Identification of New Flaviviruses Causing Reduction on Egg-laying in Chicken[J].Fujian Journal of Agricultural Sciences,2011,26(2):170-174.
Authors:CHEN Shi-long  CHEN Shao-ying  WANG Shao  LIN Feng-qing  JIANG Bin  CHENG Xiao-xia  HUANG Mei-qing  ZHU Xiao-li  LI Zhao-long
Institution:CHEN Shi-long1,2,CHEN Shao-ying1,WANG Shao1,LIN Feng-qing1,JIANG Bin1,CHENG Xiao-xia1,HUANG Mei-qing1,ZHU Xiao-li1,LI Zhao-long1,2(1.Institute of Animal Husbandry and Veterinary Medicine,Fijian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China,2.Fujian Animal Diseases Control Technology Development Center,China)
Abstract:Three virus strains were isolated from the diseased layers that experienced abrupt reduction in egg-laying in different regions.These isolated viruses could cause SPF chick-embryo and Muscovy duck-embryo to die with hemorrhage in embryo-livers.Healthy egg-producing layers inoculated with these isolates showed symptoms similar to that of the field cases,and the same viruses could be reisolated.These isolates could proliferate in MDEF causing cytopathic effect.They showed no haemagglutination activity,were se...
Keywords:layers  egg-laying reduction  new Flavivrus strain  isolation  identification  
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