| 水稻T-DNA插入启动子捕获系的初步筛选 |
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| 引用本文： | 胡昌泉,刘华清,李素一,潘大仁,王锋.水稻T-DNA插入启动子捕获系的初步筛选[J].福建农业学报,2011,26(2):143-147. |
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| 作者姓名： | 胡昌泉 刘华清 李素一 潘大仁 王锋 |
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| 作者单位： | 福建农林大学生命科学学院;福建省农业科学院生物技术研究所福建省农业遗传工程重点实验室; |
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| 基金项目： | 福建省科技计划——省属公益类科研院所基本科研专项(2009R10035-3); 福建省自然科学基金项目(2010J01099) |
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| 摘 要： | 利用农杆菌介导法将含报道基因β-glucuronidase(GUS)无启动子的转化载体pCAMBIA1300GUSAHyg导入籼稻明恢86获得水稻启动子捕获系.水稻启动子捕获系潮酶素抗感分离比χ2分析表明:39个水稻启动子捕获系中有28个为单T-DNA位点插入.2 653个水稻启动子捕获系报告基因GUS表达检测结果表明...
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| 关 键 词： | 水稻 启动子捕获系 T-DNA标签 |
Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging |
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| HU Chang-quan,LIU Hua-qing,LI Su-yi,PAN Da-ren,WANG Feng.Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging[J].Fujian Journal of Agricultural Sciences,2011,26(2):143-147. |
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| Authors: | HU Chang-quan LIU Hua-qing LI Su-yi PAN Da-ren WANG Feng |
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| Institution: | HU Chang-quan1,2,LIU Hua-qing 2,LI Su-yi2,PAN Da-ren1,WANG Feng2(1.Life Sciences College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350013,China,2.Key Laboratory of Agricultural Genetic Engineering,Fujian Academy of Agricultural Sciences,Fujian 350003,China) |
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| Abstract: | Binary vector pCAMBIA1300GUSA-Hyg containing β-glucuronidase(GUS) without the promoter was introduced into Oryza sativa L.Minghui 86 by agrobacterium-mediated transformation.The χ2 tests on the hygromycin resistant ratio revealed that 28 of the 39 rice promoter trap lines contained a loci T-DNA tagging.GUS histochemical assay at different stages in different organs,such as root,stem,leave,anther,hull,embryo and emdospern,of 2 653 rice promoter trap lines was performed.The results showed that 83 rice promoter lines were GUS positive.Of all tested,24 lines were expressed in endosperm(i.e.,0.91%) and 19 lines expressed in anther(i.e.,0.72%).Information collected on the rice promoter trap lines could be useful in identifying new rice genes. |
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| Keywords: | Rice promoter trap lines T-DNA tagging |
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