Assessment of techniques for screening alfalfa cultivars for aluminum tolerance |
| |
| Authors: | Xue-Bo Pan Cheng Zhu Chang Cheng |
| |
| Institution: | (1) State Key Lab of Plant Physiology & Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China |
| |
| Abstract: | Screening toxicity tests are an effective method to characterize aluminum tolerant plants. In this paper, three screening
procedures were used to assess aluminum tolerance among 13 cultivars of alfalfa (Medicago sativa L.). The procedures used were: Petri dish screening, black glass plot method and the soil-on-agar procedure. Major biological
indices used to analyze aluminum tolerance were relative root length (RRL), relative germination rate (RGR), relative hypocotyl
length (RHL), relative fresh weight (RFW) and root emergence (RE). Aluminum negatively affected all five indices. A Pearson
correlation test indicated that the results of the three screening procedures were consistent, suggesting that all three could
be used for screening purposes. However, because of the short test period and the simplicity of operation, the black glass
plot method might be the best choice. Overall, cv. Super No. 7 and WL-525HQ were the most aluminum-tolerant alfalfa cultivars,
while Pondus S was the most sensitive. |
| |
| Keywords: | Alfalfa Aluminum tolerance Screening procedure Soil-on-agar |
| 本文献已被 SpringerLink 等数据库收录！ |
|
