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2,3-BD多次诱导对匍匐翦股颖抗褐斑病的效果
引用本文:刘兴菊,马源,马晖玲,余倩倩.2,3-BD多次诱导对匍匐翦股颖抗褐斑病的效果[J].草原与草坪,2017(6):65-71,77.
作者姓名:刘兴菊  马源  马晖玲  余倩倩
作者单位:1. 甘肃农业大学 草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃 兰州,730070;2. 华南农业大学园艺学院,广东 广州,510642
摘    要:试验研究了250μmol/L的2,3-BD分别进行1、2、3、4次诱导对匍匐翦股颖植株抗病性的影响,计算了各处理的病情指数、诱导效果并测定了抗病相关酶以及叶绿素和丙二醛(MDA)等的含量。结果表明:2,3-BD多次诱导能使匍匐翦股颖植株的抗病性提升,其中,2次诱导处理的病情指数最低,为24.60%、诱导效果最好,为65.05%,同时与其他处理相比,,2次诱导处理下的SOD、POD、CAT、PAL等酶的活性增加,始终高于对照且差异显著,MDA的积累量最低。综合分析2,3-BD能够提高匍匐翦股颖的抗病能力,且经过2次诱导处理的效果最佳。

关 键 词:匍匐翦股颖  2  3-BD  诱导  褐斑病

Resistance effects of creeping bentgrass on brown spot disease repeatedly induced by 2,3-BD
Abstract:The brown spot caused by Rhizoctonia solani is one of the most common diseases on creeping bentgrass plants.2,3-BD (2,3-butanediol )can induce plants to improve disease resistance.The induced effects of 250 μmol/L of 2,3-BD repeatedly on creeping bentgrass plant disease resistance were investigated in this pa-per,and induction were carried out 1,2,3 and 4 times,respectively.The disease index,induction effect,disease resistant enzymes,chlorophyll and malondialdehyde content were calculated.The results showed that the re-peatedly induction of 2,3-BD improved the degree on the disease resistance of creeping bentgrass plants.The disease index was the lowest (24.6%)and induction effect was the highest (65.05%)when plants were induced 2 times.At the same time,the activities of enzymes (superoxide dismutase,peroxidase,catalase and phenylala-nine ammonia-lyase)related to disease resistance were higher than other treatments and were significantly high-er than control.Accumulation of malondialdehyde was the lowest.The 2,3-BD can improve the disease resist-ance of creeping bentgrass,and the 2 times of induction showed the best effect.
Keywords:creeping bentgrass  2  3-BD  induce  brown spot disease
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