| 氮素对紫花苜蓿根茎叶氮含量及硝酸还原酶活性的影响 |
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| 引用本文： | 于铁峰,刘晓静,张晓玲,郝凤.氮素对紫花苜蓿根茎叶氮含量及硝酸还原酶活性的影响[J].草原与草坪,2017(5):14-20. |
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| 作者姓名： | 于铁峰 刘晓静 张晓玲 郝凤 |
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| 作者单位： | 甘肃农业大学 草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃 兰州,730070 |
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| 基金项目： | 公益性行业(农业)科研专项，甘肃省科技支撑项目，国家自然科学基金 |
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| 摘 要： | 采用营养液砂培方法进行室内盆栽试验,以紫花苜蓿品种甘农3号(Medicago sativa cv.Gannong No.3)和陇东苜蓿(Medicago sativa cv.Longdong)为材料,比较研究了2种氮素形态(铵态氮、硝态氮)和5个氮素水平(0、105、210、315、420 mg/L)对现蕾期紫花苜蓿各部位氮含量及硝酸还原酶活性(NR)的影响。结果表明:2种氮素形态均能显著提高紫花苜蓿根茎叶中硝态氮含量,铵态氮含量、全氮含量及硝酸还原酶活性,且随着氮水平的提高呈先增大后减小的趋势,在210 mg/L处理达峰值。从氮素形态分析,铵态氮肥有利于铵态氮含量的增加,且根茎叶中铵态氮含量、全氮含量在铵态氮210 mg/L处理达到最大值,且显著高于其他处理;硝态氮肥有利于硝态氮含量的增加,且根茎叶中硝态氮含量、硝酸还原酶活性(NR)在硝态氮210 mg/L处理达到最大值,且显著高于其他处理。各部位中,相同氮素形态下,根茎叶中铵态氮含量、硝态氮含量、全氮含量及NR活性均表现为叶片根部茎部,且甘农3号表现优于陇东苜蓿。基于经济效益和生态效益考虑,铵态氮210 mg/L是培养紫花苜蓿较好的形态和水平。
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| 关 键 词： | 铵态氮 硝态氮 紫花苜蓿 硝酸还原酶活性 |
Effects of nitrogen on nitrogen content and nitrate reductase activity in root stem and leaf of alfalfa |
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| Abstract: | The nitrogen content and nitrate reductase activity (NR) in alfalfa organs were studied based on 2 nitrogen forms (nitrate nitrogen and ammonium nitrogen) and 5 nitrogen levels (0,105,210,315,420 mg/L). Two cultivars,Medicago sativa cv.Gannong No.3 and Medicago sativa cv.Longdong,were selected as materi-als at squaring stage,and the experiment was conducted outdoors (in flashing net room) using sand culture method.The results showed that the content of NO3 --N,the content of NH4 +-N and total nitrogen content in root and stem and leaf of alfalfa under two nitrogen treatments were significantly higher (P<0.05),and showed a trend of increased first and then decreased with the increase of nitrogen level,peaked at 210 mg/L.The content of NO3 --N,the content of NH4 +-N and total nitrogen content in root,stem and leaf of alfalfa under 2 nitrogen treatments were significantly higher (P<0.05),and showed a trend of increased first and then decreased with the increase of nitrogen level,peaked at 210 mg/L.NH4 +-N was beneficial to content of NH4 +-N,The content of NH4 +-N and total nitrogen content reached peak and significantly higher than other treatments (P<0.05) at the NH4 +-210;The content of NO3 --N and the nitrate reductase activity reached peak and significantly higher than other treatments (P<0.05) at the NO3 --210;Analysis of organ,the maximum of the contents of ammoni-um nitrogen,nitrate nitrogen,total nitrogen and NR were found at leaves,minimum in stem under the same ni-trogen form,and Gannong No.3 was better than that of Longdong.Based on the economic benefit and ecological benefit,the NH4 +-210 was better. |
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| Keywords: | NH4 +-N NO3--N alfalfa nitrate reductase activity |
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