| 几种天然抗氧化剂清除自由基能力的比较研究 |
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| 引用本文： | 梁云,王洪新.几种天然抗氧化剂清除自由基能力的比较研究[J].安徽农业科学,2008,36(6):2181-2183. |
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| 作者姓名： | 梁云 王洪新 |
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| 作者单位： | 1. 江南大学食品科学与技术国家重点实验室,江苏无锡,214122
2. 江南大学食品科学与技术国家重点实验室,江苏无锡,214122;石河子大学食品学院,新疆石河子,832003 |
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| 摘 要： | 目的]寻求比较几种天然抗氧化剂清除体外自由基能力的新方法。方法]以TB、HQ为对照,用DPPH法、邻二氮菲-Fe2+分光光度法和邻苯三酚自氧化法比较天然抗氧化剂Vc、VE、TP和迷迭香提取物对有机DPPH.、OH.及超氧自由基O2-.的清除能力。结果]试验条件下TP对DPPH.和OH.的清除能力强于Vc、VE和迷迭香提取物,而Vc对超氧自由基O2-.的清除效果较好。DPPH法较其他两种方法更适于比较这几种天然抗氧化剂清除自由基的能力。结论]DPPH法可用于比较Vc、VE、TP、迷迭香提取物和TBHQ清除DPPH.的能力。
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| 关 键 词： | 天然抗氧化剂 自由基 DPPH. 连苯三酚 |
| 文章编号： | 0517-6611(2008)06-02181-03 |
| 修稿时间： | 2007年10月22 |
Comparative Study on the Abilities of Scavenging Free Radical of Some Natural Antioxidants |
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| LIANG Yun.Comparative Study on the Abilities of Scavenging Free Radical of Some Natural Antioxidants[J].Journal of Anhui Agricultural Sciences,2008,36(6):2181-2183. |
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| Authors: | LIANG Yun |
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| Abstract: | Objective] The purpose of the study was to seek for the new method of comparing the abilities of scavenging free radical in vitro of some natural antioxidants.Method] With TBHQ as CK,the abilities of scavenging organic DPPH·,OH· and superoxide radical O2-· of natural antioxidants such as Vc,VE,TP and rosemary extract were compared by DPPH,Phenanthroline-Fe2+ spectrophotometry and pyrogallol autoxidation methods.Result] Under the experimental condition,the ability of scavenging DPPH· and OH· of TP was stronger than that of Vc,VE and rosemary extract,but Vc had better scavenging effect on superoxide radical O2-·.DPPH method was more suitable for comparing the abilities of scavenging free radical of these natural antioxidants than the other 2 methods.Conclusion] DPPH method could be used to compare the abilities of scavenging DPPH· of Vc,Ve,TP,rosemary extract and TBHQ. |
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| Keywords: | Natural antioxidant Free radical DPPH· Pyrogallol |
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