| 伽马辐照对智利小植绥螨生殖及性别决定的影响 |
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| 引用本文： | 张保贺,王恩东,吕佳乐,徐学农.伽马辐照对智利小植绥螨生殖及性别决定的影响[J].中国生物防治学报,2016,32(6):681-688. |
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| 作者姓名： | 张保贺 王恩东 吕佳乐 徐学农 |
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| 作者单位： | 中国农业科学院植物保护研究所, 北京 100193 |
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| 基金项目： | 国家“973”计划(2013CB1276024) |
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| 摘 要： | 辐照可导致染色体变异，是研究生殖机理的常用辅助方法之一。本研究对不同生理状况下智利小植绥螨雌螨、雄螨、不同发育阶段卵进行不同剂量的伽马辐照，探索其后代发育与繁殖情况，以期解释智利小植绥螨性别决定机制及生殖机理。研究结果表明，在160~640 Gy辐照剂量下，辐照的未交配的雄成螨与正常的未交配的雌成螨交配，F1代中只有雄成螨且仅1/3可育。在45~90 Gy辐照剂量下，辐照的未交配的雌成螨与正常的未交配的雄成螨交配，其子代雌性比随着辐照剂量的增加而减小，且至90 Gy处理下无子代完成胚胎发育。辐照交配后产卵前的雌成螨，其子代雌性比间没有显著差异（77.5%~85.0%）。辐照卵龄0~12 h的卵，没有卵发育至成螨；辐照卵龄12~24 h的卵，其中发育至成螨的全为雌螨；辐照卵龄24~48 h的卵，其中发育至成螨的雌性比随着辐照剂量的增加具有增加的趋势。试验结果推测出父系染色体组参与形成配子且对性别起决定作用；父系染色体组异质化的时间可能集中于卵壳形成后至卵期12 h之间。本试验为智利小植绥螨父系染色体组参与性别决定的机制做出了新的诠释，对深入研究性别决定的机理具有重要的意义。
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| 关 键 词： | 智利小植绥螨 伽马射线 生殖 性别决定 |
| 收稿时间： | 2016-05-04 |
Impact of Gamma Irradiation on Reproduction and Sex Determination in Phytoseiulus persimilis Athias-Henriot |
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| ZHANG Baohe,WANG Endong,L&#,Jiale,XU Xuenong.Impact of Gamma Irradiation on Reproduction and Sex Determination in Phytoseiulus persimilis Athias-Henriot[J].Chinese Journal of Biological Control,2016,32(6):681-688. |
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| Authors: | ZHANG Baohe WANG Endong L&# Jiale XU Xuenong |
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| Institution: | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | Irradiation is an important tool for chromosome aberration, and is often used in investigating reproduction mechanisms of insects and mites. In this study, we investigated the impact of gamma irradiation on female adults, male adults and eggs of different ages of Phytoseiulus persimilis Athias-Henriot. When irradiated males (at 160-640 Gy) were mated with unmated females, all F1 offspring were males and only 1/3 were fertile. When irradiated females (at 45-90 Gy) were mated with unmated males, the proportion of females in F1 offspring decreased with increasing irradiation dose, and no offspring were able to complete embryonic development when the irradiation dose was 90 Gy. No significant impact on proportion of female (0.78-0.85) in offspring was observed when fertilized females were irradiated during preoviposition stage. When 0-12 h old eggs were irradiated, no larva emerged. When 12-24 h old eggs were irradiated, all individuals that completed development to adult stage were females. When 24-48 h old eggs were irradiated, proportion of female increased with increasing irradiation dose. Our study shows that paternal chromosome is involved in sex determination in offspring. Possible timing of paternal chromosome heterogenization is between egg shell formation and 12 h after oviposition. This study provides new explanations for the mechanism of offspring sex determination and the role played by paternal chromosome in P. persimilis, which is important for further study of reproductive mechanisms of predatory mites. |
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| Keywords: | Phytoseiulus persimilis gamma rays reproduction sex determination |
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