| 苏云金芽胞杆菌JQ23的表型鉴定及对韭菜迟眼蕈蚊的防治效果 |
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| 引用本文： | 宋健,曹伟平,张海剑,王金耀,冯书亮,杜立新.苏云金芽胞杆菌JQ23的表型鉴定及对韭菜迟眼蕈蚊的防治效果[J].中国生物防治学报,2016,32(3):326-331. |
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| 作者姓名： | 宋健 曹伟平 张海剑 王金耀 冯书亮 杜立新 |
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| 作者单位： | 河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 |
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| 基金项目： | 国家公益性行业(农业)科研专项(201303027) |
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| 摘 要： | 韭菜迟眼蕈蚊是韭菜生产上的重要害虫,实验室从河南省采集的土样中分离获得1株对韭菜迟眼蕈蚊幼虫高毒力的Bt新菌株JQ23。经BiologGENⅢ细菌鉴定系统分析菌株JQ23为苏云金芽胞杆菌,相似率为0.596;通过室内生物活性测定,明确菌株JQ23对韭菜迟眼蕈蚊2龄幼虫72h的LC50值为8.38×106芽胞/mL;经室内盆栽防治试验结果表明,菌株JQ23在1×107芽胞/mL浓度下,采用滴灌法连续用药3次,间隔7d,对韭菜迟眼蕈蚊的防治效果达到86.28%;经春季田间小区试验,再次验证菌株JQ23在1×108芽胞/mL浓度下,采用滴灌法连续用药3次,间隔7~10d,对韭菜迟眼蕈蚊的防治效果达到66.75%。由此可见,菌株JQ23能够有效地控制韭菜迟眼蕈蚊的虫口数量,减少作物的受害率,达到较好的防治效果。
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| 关 键 词： | 韭菜迟眼蕈蚊 苏云金芽胞杆菌 防治效果 |
| 收稿时间： | 2015-09-18 |
The Phenotype Determination of Bacillus thuringiensis JQ23 and Its Control Efficiency against Bradysia odoriphaga |
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| SONG Jian,CAO Weiping,Zhang Haijian,Wang Jinyao,Feng Shuliang,DU Lixin.The Phenotype Determination of Bacillus thuringiensis JQ23 and Its Control Efficiency against Bradysia odoriphaga[J].Chinese Journal of Biological Control,2016,32(3):326-331. |
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| Authors: | SONG Jian CAO Weiping Zhang Haijian Wang Jinyao Feng Shuliang DU Lixin |
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| Institution: | Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China |
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| Abstract: | Bradysia odoriphaga is an important pest of Chinese chive production. A new Bacillus thuringiensis strain JQ23 was isolated from the soil collected from Henan province by antibiotic method. GEN III Biolog bacterial identification system showed that JQ23 strain can use 13 kinds of carbon sources, and is sensitive to 9 chemical substances. Bioassay showed that JQ23 strain was of high insecticidal activity against B. odoriphaga, with 72 h LC50 of 8.3804×106 spore/mL against 2nd instars of B. odoriphaga. The pot tests showed that, continuous dripping application of JQ23 at 1×107 spore / mL for three times at intervals of 7 d, the final control reached 86.28%. In field plot experiment in spring, continuous dripping application of JQ23 at 1×108 spore/mL for three times at intervals of 7―10 d, the final control was 66.75%. These results show that the JQ23 strain can achieve an effective control of B. odoriphaga. |
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| Keywords: | Bradysia odoriphaga Bacillus thuringiensis control effect |
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