| 对斜纹夜蛾高致病性莱氏野村菌Nr19固体培养条件的优化 |
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| 引用本文： | 唐维媛,邢丛丛,董永刚,王雪郦,张义明.对斜纹夜蛾高致病性莱氏野村菌Nr19固体培养条件的优化[J].中国生物防治学报,2016,32(2):180-188. |
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| 作者姓名： | 唐维媛 邢丛丛 董永刚 王雪郦 张义明 |
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| 作者单位： | 1. 贵州大学昆虫研究所, 贵阳 550025;
2. 贵州大学发酵工程与生物制药省级重点实验室, 贵阳 550025;
3. 贵州大学酿酒与食品工程学院, 贵阳 550025;
4. 贵州大学化学与化工学院, 贵阳 550025;
5. 贵州工业职业技术学院, 贵阳 550008 |
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| 基金项目： | 贵州省科学技术基金项目([2010]2062、[2012]2101)，贵州省农业攻关项目([2013]3045) |
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| 摘 要： | 经前期生物测定,莱氏野村菌Nomuraea rileyi(Nr19)菌株能使斜纹夜蛾Spodoptera litura(Fabricius)幼虫大量感病及死亡,为了提高分生孢子产量,本文采用单因素试验,对影响菌株Nr19生长的固体发酵工艺条件进行了优化。结果表明,大豆与麦麸混合物是菌株Nr19的理想发酵基质,适量麸皮的加入,能有效提高产孢量。培养期间温湿度的分段控制对产孢的影响也较大。优化后的培养条件为:大豆、麸皮混合比例为85:15,液体种子种龄5 d,接种量15.0%,连续光照,期间控制前3 d相对湿度为85%,之后降为60%,而培养温度则采用前2 d与后11 d保持在25℃,中间升温至28℃的分段控温方式培养16 d。此条件下产孢量比优化前提高近2倍,为(6.045±0.061)×109孢子/g,而活孢率可达(93.770±0.278)%,可为后续高侵染力杀虫制剂的研究提供较充足的试验材料。
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| 关 键 词： | 莱氏野村菌 固体发酵工艺 温湿度 产孢量 活孢率 |
| 收稿时间： | 2015-05-13 |
Optimization of Solid Culture Conditions for Nomuraea rileyi (Nr19) a Pathogenic Fungus of Spodoptera litura |
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| TANG Weiyuan,XING Congcong,DONG Yonggang,WANG Xueli,ZHANG Yiming.Optimization of Solid Culture Conditions for Nomuraea rileyi (Nr19) a Pathogenic Fungus of Spodoptera litura[J].Chinese Journal of Biological Control,2016,32(2):180-188. |
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| Authors: | TANG Weiyuan XING Congcong DONG Yonggang WANG Xueli ZHANG Yiming |
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| Institution: | 1. Institute of Entomology, Guizhou University, Guiyang 550025, China;
2. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang 550025, China;
3. College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;
4. College of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China;
5. Guizhou Industry Polytechnic College, Guiyang 550008, China |
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| Abstract: | The solid culture conditions were optimized using single factor experiment, with the aim to improve the conidium production of a strain of Nomuraea rileyi (Nr19) that was determined to be pathogenic to Spodoptera litura larvae. The results showed that the mixture of soybean and wheat bran is a good fermentation medium for Nr19 and significant increase of yields could be achieved with adequate addition of the mixture bran. Control of stage-specific temperature and relative humidity influenced the culture process as well. The optimized conditions were:soybean:bran=85:15, spawn age=5 d, inoculum=15.0%, photoperiod=24L:0D, relative humidity=85% in first 3 d and 60% thereafter, temperature=25℃ in the first 2 d and 28℃ for 3 d and then 25℃ for the last 11 d. Under this optimal fermentation conditions, the highest yields of spore reached (6.045±0.061)×109 conidia/g, which was nearly three folds of that with the un-optimized conditions, and the conidia viability was (93.770±0.278)%. These results can provide practical and experimental supports for further research on preparation of biocontrol agents. |
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| Keywords: | Nomuraea rileyi solid culture condition optimization temperature and humidity yields of spore conidial viability |
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