| 琼枝野生群体与养殖群体的EST-SSR分析 |
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| 引用本文： | 胡吟胜,段泽林,黄 勃,于淑楠,王 婷.琼枝野生群体与养殖群体的EST-SSR分析[J].水产学报,2013,37(9):1313-1318. |
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| 作者姓名： | 胡吟胜 段泽林 黄 勃 于淑楠 王 婷 |
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| 作者单位： | 海南大学海洋学院;华东师范大学生命科学学院;海南大学海洋学院;海南大学海洋学院;中国科学院海洋研究所 |
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| 基金项目： | 国家“八六三”高技术研究发展计划(2012AA10A412-8);海南大学植物学国家重点学科(071001);海南省重点科技项目(080137);国家自然科技基金项目(41176084);国家科技支撑计划项目(2009BADB2B0404-02);海洋公益行业科研专项(201105008-7);中医药行业科研专项(201207002-03) |
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| 摘 要： | 为研究野生琼枝与养殖琼枝的遗传差异，利用EST-SSR技术分析了海南东北部地区9株野生和9株养殖琼枝的遗传差异性。结果显示，用5对EST-SSR引物对18个个体的基因组进行扩增，扩增出的片段大小为250～2 500 bp，琼枝野生群体和养殖群体的多态性比例分别为74.29%和70.00%；野生群体之间的平均遗传距离为0.46，养殖琼枝群体之间的平均遗传距离为0.22。聚类分析图显示，在相似系数0.49处，18株群体按野生与养殖分为2大类，在相似系数0.66附近，野生群体分成3类，养殖琼枝群体分为2类，在相似系数0.73附近，野生琼枝群体分成5类，而养殖群体为3类，相似系数越高，野生群体比养殖群体分类单元越多。研究表明，野生琼枝群体的遗传多样性比养殖琼枝群体高。
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| 关 键 词： | 琼枝 EST-SSR 野生 养殖 |
| 收稿时间： | 2013/3/24 0:00:00 |
| 修稿时间： | 2013/4/27 0:00:00 |
EST-SSR analysis of wild and farmed Betaphycus gelatinum |
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| HU Yinsheng,DUAN Zelin,HUANG Bo,YU Shunan and WANG Ting.EST-SSR analysis of wild and farmed Betaphycus gelatinum[J].Journal of Fisheries of China,2013,37(9):1313-1318. |
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| Authors: | HU Yinsheng DUAN Zelin HUANG Bo YU Shunan and WANG Ting |
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| Abstract: | EST-SSR was used to detect the hereditary difference in wild and cultured populations of the Betaphycus gelatinae,the result showed that nine individuals from each population were used of the 5 EST-SSR primers amplified,a total of 195 reproducible loci ranging from 250 to 2 500 bp were amplified from all the 18 individuals.The average percentages of polymorphic loci in wild and cultured populations were 74.29% and 70%.The intra-population genetic distance was 0.46 in wild population and 0.22 in cultured population.The cluster analysis showed that 18 individuals were clustered into two groups base on 0.49 of GS; wild populations were divided into 3 branches and cultured populations were divided into 2 branches base on 0.66; wild populations were divided into 5 branches and cultured populations were divided into 3 branches base on 0.73.The higher the value,the more branches the wild populations have.The research shows that genetic diversity of wild populations ampler than cultured. |
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| Keywords: | Betaphycus gelatinae EST-SSR wild farmed |
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