| 怀山药茎尖脱毒培养与茎段增殖研究 |
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| 引用本文： | 苗利娟,韩锁义,张新友,黄冰艳,汤丰收,董文召,王素霞.怀山药茎尖脱毒培养与茎段增殖研究[J].河南农业科学,2011,40(11):123-125. |
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| 作者姓名： | 苗利娟 韩锁义 张新友 黄冰艳 汤丰收 董文召 王素霞 |
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| 作者单位： | 1. 河南省农业科学院经济作物研究所,河南郑州,450002
2. 温县农业局,河南温县,454850 |
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| 基金项目： | 国家公益性行业科研专项 |
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| 摘 要： | 以怀山药的茎尖、茎段为外植体,研究不同激素配比对山药茎尖分化及茎段快繁的影响,筛选适合于怀山药生长的培养基.结果表明:MS+BA 1 mg/L+KT 2mg/L+NAA 0.2mg/L是怀山药茎尖分化较理想的培养基,成苗率较高(30％)；MS+BA 1 mg/L +NAA 0.5mg/L是怀山药茎段快繁最佳培养基,不定...
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| 关 键 词： | 怀山药 茎尖 茎段 培养基 分化 脱毒 快繁 |
The Establishment of the System for Detoxified Huai Yam by Stem Tip Culture and Stem Cut Rapid Propagation |
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| MIAO Li-juan,HAN Suo-yi,ZHANG Xin-you,HUANG Bing-yan,TANG Feng-shou,DONG Wen-zhao,WANG Su-xia.The Establishment of the System for Detoxified Huai Yam by Stem Tip Culture and Stem Cut Rapid Propagation[J].Journal of Henan Agricultural Sciences,2011,40(11):123-125. |
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| Authors: | MIAO Li-juan HAN Suo-yi ZHANG Xin-you HUANG Bing-yan TANG Feng-shou DONG Wen-zhao WANG Su-xia |
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| Institution: | 1.Industrail Crops Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.Agricultural Bureau of Wen County,Wen County 454850,China) |
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| Abstract: | The effects of different hormone combinations on yam shoot tip differentiation and propagation were studied using shoot tip and stem cut as explants,to select the medium suited for Huai yam culture.The result showed that MS+BA 1 mg/L+KT 2 mg/L+NAA 0.2 mg/L was an ideal medium for Huai yam tip differentiation with its high rate of seedling production(30%).MS+BA 1 mg/L+NAA 0.5 mg/L was the best medium for rapid propagation by Huai yam stem cuts with its relatively high induction rate of adventitious buds and highest rate of seedling production(109.5%).Addition of 0.01% PVP inhibited browning remarkably. |
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| Keywords: | Huai yam Stem tip Stem cut Medium Differentiation Detoxification Rapid propagation |
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