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Effect of Antibiotic-containing Extenders on Taylorella equigenitalis Contaminated Semen |
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| Authors: | Bettina T Olivieri Brenda C Love Grant B Rezabek Catherine G Lamm Dickson D Varner Mark E Payton G Reed Holyoak |
| Institution: | a Department of Veterinary Clinical Science, Center for Veterinary Health Sciences, Stillwater, OK b Oklahoma Animal Disease Diagnostic Laboratory, Center for Veterinary Health Sciences, Stillwater, OK c Department of Large Animal Clinical Sciences, Center for Veterinary Medicine, College Station, TX d Department of Statistics, Oklahoma State University, Stillwater, OK |
| Abstract: | Taylorella equigenitalis is a gram-negative coccobacillus and the causative agent of a transmissible venereal disease in horses known as contagious equine metritis. Outbreaks of contagious equine metritis have been documented in various countries since 1977, with the most recent discovery in the United States in December 2008. During disease occurrences, culturing semen samples for T equigenitalis before breeding may help to prevent transmission of this disease; however, little is known about the antimicrobial activity of equine semen extenders against the organism. The purpose of this study was to investigate the infectivity levels of T equigenitalis in three equine semen extenders inoculated with known concentrations of the organism. The semen extenders used for this study included INRA 96, E-Z Mixin BF, and VMDZ. In addition, Timentin was added to INRA 96 at three different concentrations (0.5, 1.0, and 1.5 mg/mL) to investigate possible synergistic effects of antibiotic supplementation of extenders. Results were based on the visual counting of the colonies on chocolate Eugon agar plates. Both INRA 96 (with added Timentin) and VMDZ (as supplied by the manufacturer) significantly reduced the numbers of T equigenitalis isolated from semen extenders as compared with INRA 96 (as supplied by the manufacturer) or the antibiotic free E-Z Mixin BF. Our findings indicate that INRA 96 (with added Timentin) or VMDZ may significantly decrease the growth of T equigenitalis in extended semen; however, it is also important to consider the possible effects of antibiotic supplemented extenders on sperm longevity and fertility in addition to eliminating specific pathogens in semen. |
| Keywords: | Taylorella equigenitalis Contagious equine metritis INRA 96 E-Z Mixin BF Basic formula VMDZ Semen extender Timentin |
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