| 小麦高分子量谷蛋白1Dx5亚基特异PCR标记 |
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| 引用本文： | 赵辉,闫华晓,王宪泽.小麦高分子量谷蛋白1Dx5亚基特异PCR标记[J].山东农业大学学报(自然科学版),2004,35(1):22-26. |
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| 作者姓名： | 赵辉 闫华晓 王宪泽 |
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| 作者单位： | 山东农业大学生命科学学院,山东,泰安,271018 |
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| 基金项目： | 山东省30工程项目部分研究内容 |
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| 摘 要： | 本研究为1Dx5亚基基因(Glu—Dl-1b)设计了一对特异引物，对HMW—GS在Glu—Dx1位点已知的11个小麦品种进行了PCR扩增，以检测PCR标记的准确性。结果表明，凡具有1Dx5亚基的4个品种都能扩增出一条450bp的特异带，而其它品种则不能扩增出这一特异带．与HMW—GS的蛋白质电泳结果一致。用这一特异标记对山东省推广种植面积较大的35个品种进行PCR检测．发现仅有9个品种携带1Dx5基因，占待测材料总数的25．7％，明显低于北美小麦品种。研究发现，与蛋白质的HMW—GS标记相比．PCR标记更快速、简便、准确。
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| 关 键 词： | 小麦 高分子量谷蛋白 1Dx5亚基 PCR标记 品质 |
| 文章编号： | 1000-2324(2004)01-0022-05 |
| 修稿时间： | 2003年5月7日 |
SPECIFIC PCR MARKER OF HMW-GS 1Dx5 GENE IN WHEATS |
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| ZHAO Hui,YAN Hua-xiao,WANG Xian-ze.SPECIFIC PCR MARKER OF HMW-GS 1Dx5 GENE IN WHEATS[J].Journal of Shandong Agricultural University,2004,35(1):22-26. |
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| Authors: | ZHAO Hui YAN Hua-xiao WANG Xian-ze |
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| Institution: | ZHAO Hui,YAN Hua-xiao,WANG Xian-ze * |
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| Abstract: | Specific primers for Glu-D1-1b (1Dx5) gene were designed to detect HMW-GS better. Results showed that PCR products of 450bp were amplified in 4 materials carrying the 1Dx5 subunit when 11 cultivars with HMW-GS composition known were tested. The primers were used to detect 35 wheat strains of Shandong province also. It indicated that there were 9 materials having the 1Dx5 gene, far lower than that of wheat lines of North America. Compared with SDS-PAGE detection, PCR is quicker,simple and accurate. |
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| Keywords: | wheat high molecular weight glutelin subunit PCR |
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