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Plant growth promoting properties of a strain of Enterobacter ludwigii isolated from Lolium perenne rhizosphere
Authors:Mauricio Shoebitz  Claudia M Ribaudo  Martín A Pardo  Luigi Ciampi
Institution:a Instituto de Producción y Sanidad Vegetal, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Valdivia, Chile
b Cátedra de Bioquímica, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina
c Instituto de Investigaciones Bioquímicas y Fisiológicas (IByF), CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Avenida San Martín 4453 (DSE 1417), Ciudad Autónoma de Buenos Aires, Argentina
Abstract:The capability of native bacterial strains isolated from Lolium perenne rhizosphere to behave as plant growth promoting bacteria and /or biocontrol agents was investigated. One strain (BNM 0357) over 13 isolates from the root tips of L. perenne resulted proved to be nitrogenase positive (ARA test) and an IAA producer. Conventional tests and the API 20E diagnostic kit indicated that BNM 0357 behaves to the Enterobacteriaceae family and to the Enterobacter genus. Molecular identification by 16S rRNA sequence analysis indicated that BNM 0357 had the highest similarity to Enterobacter ludwigii (EN-119). Isolate BNM 0357 had the capability to solubilize calcium triphosphate and to antagonize Fusarium solani mycelial growth and spore germination. Strain BNM 0357 also showed the ability to improve the development of the root system of L. perenne. This study disclosed features of E. ludwigii BNM 0357 that deserve further studies aimed at confirming its putative importance as a PGPR.
Keywords:Enterobacter ludwigii  Plant growth promotion  Lolium perenne  Biocontrol  Fusarium solani
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