| 一株脱氧雪腐镰刀菌烯醇转化菌的分离、鉴定及其转化效果研究 |
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| 引用本文： | 何成华,樊彦红,刘国芳,张海彬.一株脱氧雪腐镰刀菌烯醇转化菌的分离、鉴定及其转化效果研究[J].南京农业大学学报,2009,32(4). |
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| 作者姓名： | 何成华 樊彦红 刘国芳 张海彬 |
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| 作者单位： | 南京农业大学动物医学院,江苏 南京,210095 |
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| 基金项目： | 国家自然科学基金项目，教育部博士点基金项目，江苏省科技攻关项目 |
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| 摘 要： | 以脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)为唯一碳源,通过富集培养从土壤中分离到一株可以转化DON的真菌,命名为NJA-1.用引物Bt2a、Bt2b扩增了该菌的β-tubulin基因序列,GenBank的登录号为DQ902579.通过基因序列分析和形态学观察最终鉴定该菌株为塔宾曲霉(Aspergillus tubingensis).该菌与塔宾曲霉AY820009的同源性为99%.在pH 60、30 ℃、14 d内其对DON的平均转化率为944%.HPLC-MS分析结果表明,DON转化产物的相对分子质量为3144,比DON的相对分子质量(2963)增加了181.该菌在无机盐培养基中对DON的最高耐受浓度为40 mg·L-1.提示:该菌可用于DON的生物转化.
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇 生物转化 分离 鉴定 β-tubulin基因 塔宾曲霉 |
Isolation and identification of a strain of Aspergillus tubingensis with deoxynivalenol biotransformation capability |
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| HE Cheng-hua,FAN Yan-hong,LIU Guo-fang,ZHANG Hai-bin.Isolation and identification of a strain of Aspergillus tubingensis with deoxynivalenol biotransformation capability[J].Journal of Nanjing Agricultural University,2009,32(4). |
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| Authors: | HE Cheng-hua FAN Yan-hong LIU Guo-fang ZHANG Hai-bin |
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| Abstract: | A colony was isolated from soil by inorganic salt culture media added deoxynivalenol (DON) and numbered NJA - 1. Bt2a and Bt2b were used as primers to amplify the β-tubulin gene of NJA -1. The sequence analysis of Bt2a-Bt2b was submitted to GenBank. The accession number in Gen Bank was DQ902579. Sequence analysis and morphology observation indicated that NJA-1 belonged to Aspergillus tubingensis. Its homology to Aspergillus tubingensis AY820009 was 99%. The mean UON-biotransformation rate was 94. 4% after two weeks cultivation at pH 6. 0 and 30℃. The relative molecular weight of biotransformation product analyzed by HPLC-MS was 314.4, which was 18. 1 larger than that of DON (296.3). NJA - 1 could tolerate the maximum 40 mg· L~(-1) DON in inorganic salt media. The result showed that NJA - 1 could to be used for DON biotransformation. |
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| Keywords: | deoxynivalenol biotransformation isolation identification β-tuhulin gene Aspergillus tubingensis |
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