| 无患子总DNA提取方法研究 |
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| 引用本文： | 刘宝,范辉华,彭朱清.无患子总DNA提取方法研究[J].福建林业科技,2013,40(2):28-31. |
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| 作者姓名： | 刘宝 范辉华 彭朱清 |
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| 作者单位： | 1. 福建农林大学林学院,福建福州,350002
2. 福建省林业科学研究院,福建福州,350012 |
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| 基金项目： | 国家林业公益性行业科研专项“果用无患子良种选育及高效栽培技术研究”资助 |
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| 摘 要： | 针对无患子富含多酚、多糖、蛋白质及其他次生代谢物质的特点,以无患子叶片为材料,分别采用改良的CTAB法和CTAB与SDS相结合法提取不同种源无患子的总DNA。结果显示,改良的CTAB法提取的总DNA,得率较高,纯度好,可用于酶切、PCR扩增等后续试验,并能获得清晰、稳定的扩增产物。而采用CTAB与SDS相结合的方法得到的基因组DNA效果不稳定,无法检测出DNA。改良的CTAB法具有简单、经济的特点,可用于无患子总DNA的提取,成功地进行SRAP分子标记分析,为研究无患子遗传资源多样性奠定基础。
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| 关 键 词： | 无患子 DNA提取 改良CTAB法 |
Extraction of Total DNA from Sapindus Mukorossi |
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| LIU Bao,FAN Hui-hua,PENG Zhu-qing.Extraction of Total DNA from Sapindus Mukorossi[J].Journal of Fujian Forestry Science and Technology,2013,40(2):28-31. |
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| Authors: | LIU Bao FAN Hui-hua PENG Zhu-qing |
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| Institution: | 1(1.College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;2.Fujian Research Institute of Forestry,Fuzhou 350012,Fujian,China) |
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| Abstract: | In view of the large amounts of secondary metabolites such as polyphenol,polysaccharide and protein in leaves of Sapindus Mukorossi,the total DNA of different provenances were extracted by improved CTAB and CTAB combined with SDS methods.Results showed that the effect of the improved CTAB method was better,DNA yield was higher with high purity,could be used for the follow-up tests like enzyme cut,PCR amplification,could obtain clear and stable amplification product.Otherwise,the effect of CTAB combined with SDS method got genome DNA was not stable,DNA concentration was low,it always couldn't detect DNA.Improved CTAB method had a simple and economic characteristics,could be used for the Sapindus Mukorossi’s total DNA extraction,successful used to the SRAP molecular marker analysis.It’s laid the foundation for the research of Sapindus Mukorossi’s diversity of genetic resources. |
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| Keywords: | Sapindus Mukorossi DNA Extraction improved CTAB method |
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