| 三七黑斑病抗性人工鉴定方法的建立* |
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| 引用本文： | 杨涛,陈昱君,段承俐,徐绍忠,萧凤回,王勇,文国松.三七黑斑病抗性人工鉴定方法的建立*[J].云南农业大学学报,2006,21(5):549-553. |
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| 作者姓名： | 杨涛 陈昱君 段承俐 徐绍忠 萧凤回 王勇 文国松 |
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| 作者单位： | (1.云南农业大学中药材研究所,云南省中药材规范化种植指导中心, 云南省生物技术创新人才培养基地,云南 昆明 650201;;2.云南省文山州三七科学技术研究所,云南 文山 663000) |
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| 摘 要： | 三七黑斑病(Alternaria panax Whetz)是影响三七生产尤其是GAP规范化生产的重要病害,建立三七黑斑病抗性人工鉴定方法是开展三七抗病育种必备环节。分别以室内离体叶片和田间在体叶片进行不同接种方法的试验,明确了通过针刺叶片接种浓度为1.0×107个/mL的三七黑斑病孢子悬浮液是诱导发病有效途径。室内离体接种和田间接种对比,同一植株室内离体叶片接种与田间接种的相关系数0.9548**,达到极显著水平。对个体基因型还有变异的三七栽培群体进行人工抗性鉴定的初步研究发现,不同个体三七对黑斑病接种的发病程度有明显的差异,说明黑斑病抗病性与三七个体遗传特性有关。测定田间接种发病程度不同的三七叶片的苯丙氨酸解氨酶活性,发现三七对黑斑病的抗性与三七植株叶片苯丙氨酸解氨酶活性有明显的关系。
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| 关 键 词： | 三七 黑斑病 人工接种鉴定 抗病性 苯丙氨酸解氨酶 |
| 收稿时间： | 2006-1-17 |
The Methodology for Artificial Identification of Panax notoginseng Resistance to Black Spot Disease |
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| YANG Tao,CHEN Yu-jun,DUAN Cheng-li,XU Shao-zhong,XIAO Feng-hui,WANG Yong,WEN Guo-song.The Methodology for Artificial Identification of Panax notoginseng Resistance to Black Spot Disease[J].Journal of Yunnan Agricultural University,2006,21(5):549-553. |
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| Authors: | YANG Tao CHEN Yu-jun DUAN Cheng-li XU Shao-zhong XIAO Feng-hui WANG Yong WEN Guo-song |
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| Institution: | (1.Institute of Chinese Medical Materials of Y A U, Yunnan Provincial Center for Chinese Medicinal Materials, GAP Technology, Center for Agriculture and Biodiversity Research and Training of Yunnan Province, Kunming 650201, China;2.Wenshan Prefectural P.notoginseng Science and Technology Research Institute, Wenshan 663000, China) |
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| Abstract: | Black spot disease (Alternaria panax Whetz) is one of the most severe diseases which impacts on the production and Good Agricultural Practice of Panax notoginseng (Burk) CHEN F H. It is an essential step to set up a methodology for artificial identification of Panax notoginseng resistance to black spot disease in its disease resistance breeding. By using leaves in vitro at room and ones in vivo at field to conduct several kinds of artificial infection experiments, it has been found that inoculation of the spore suspension with concentration of 1.0×107 spore/mL on the leaves which had been punctured with needle was an effective method to induce the disease. The comparison between methods in vitro and in vivo showed that there was a significant correlation between them (coefficients was 0.9548**). The artificial identification of cultivated population consisting of genetically unidentical individuals in field for notoginseng resistance to the disease indicated that there was outstanding variation in disease infection scale among the plant individuals, which suggested the disease resistance might have something to do with the individual genotype. Through test of activity of phenylalanine ammonium-lyase in notoginseng plants displaying various resistance to black spot disease in the filed, we found the occur of clear relation between the activity of the enzyme and the disease resistance of the plants. |
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| Keywords: | Panax notoginseng" target="_blank">Panax notoginseng')" href="#">Panax notoginseng Alternaria panax Whetz" target="_blank">Alternaria panax Whetz')" href="#">Alternaria panax Whetz artificial infection idenfication disease resistance phenylalanine ammonium-lyase |
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