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| 生防细菌SF-16产芽胞条件的研究 | |
| 引用本文： | 张明,李欢,朱莹.生防细菌SF-16产芽胞条件的研究[J].畜牧与饲料科学,2012,33(9):28-30. |
| 作者姓名： | 张明 李欢 朱莹 |
| 作者单位： | 1. 河北联合大学冀唐学院,河北唐山,063000 2. 河北省唐山市人民医院检验科,河北唐山,063000 3. 河北联合大学医学实验研究中心,河北唐山,063000 |
| 基金项目： | 河北联合大学青年基金项目 |
| 摘 要： | 生防细菌SF-16为土壤中筛选得到的1株细菌,研究表明,其对多种植物致病真菌有抑制作用。为了提高生防细菌SF-16的芽胞形成率及芽胞数量,以及为工厂化生产生物菌剂提供理论依据,采用摇瓶发酵对芽胞形成的主要影响因素进行了考察。通过单因素实验和正交实验对SF-16菌株产芽胞条件进行了分析,确定了摇瓶发酵最佳条件为:乳糖0.5%、黄豆饼粉2.0%、MnSO4·5H2O0.30%。在该优化条件下,发酵液中颉颃菌的出芽率达到98.3%,最终生物量达到2×109个/mL。 |
| 关 键 词： | 生防细菌 芽胞 发酵条件 SF-16 |
Studies on Biocontrol Bacteria SF-16 Spore Production Condition |
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| ZHANG Ming,LI Huan,ZHU Ying.Studies on Biocontrol Bacteria SF-16 Spore Production Condition[J].Animal Husbandry and Feed Science,2012,33(9):28-30. | |
| Authors: | ZHANG Ming LI Huan ZHU Ying |
| Institution: | 1.Jitang College,Hebei Union University,Tangshan 063000,China;2.Clinical Laboratory of Tangshan People＇s Hospital,Tangshan 063000,China;3.Medical Experimental Research Center of Hebei Union University,Tangshan 063000,China) |
| Abstract: | Biocontrol bacteria SF-16 was a selected bacteria strain,research showed that its inhibitory effect on multiple plants.In order to improve biocontrol bacteria SF-16 spore formation rate and quantity,provide theoretical basis for factory production of biological agent,use flask fermentation with a survey on main influencing factors of spore formation.Through single factor experiment and orthogonal experiment,the optimal shaking flask fermentation condition was determined as follow：media composed of 0.5% lactose,2.0% soybean meal,0.30% MnSO4·5H2O.Under this optimum condition,the germination rate achieves 98.3 percentages,Biomass achieves 2×109/mL. |
| Keywords: | biocontrol bacteria spore fermentation condition SF-16 |
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