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| 河南地方野生小鼠与近交系小鼠的RAPD分析 | |
| 引用本文： | 雷建,华欣欣,周鑫,柴保国,赵卫东,郑振宇.河南地方野生小鼠与近交系小鼠的RAPD分析[J].河南农业科学,2010(5). |
| 作者姓名： | 雷建 华欣欣 周鑫 柴保国 赵卫东 郑振宇 |
| 作者单位： | 1. 河南农业大学,牧医工程学院,河南,郑州,450002 2. 河南农业大学,生命科学学院,河南,郑州,450002 3. 河南农业大学,牧医工程学院,河南,郑州,450002;河南农业大学,生命科学学院,河南,郑州,450002 |
| 基金项目： | 转基因生物新品种培育科技重大专项，河南省科技创新杰出人才计划项目 |
| 摘 要： | 选用120个随机引物,对河南地方野生小鼠及其他4个近交系小鼠品系进行RAPD分析,筛选出36个多态性引物,检测到380条扩增片段,其中多态性片段304条,且野生小鼠具有若干特异条带,反映出野生小鼠与4个近交系小鼠品系间存在遗传差异。多态性分析表明,野生小鼠与PWK之间的遗传距离指数为0.009 24,遗传关系最近;与DDK遗传距离指数为0.011 30,遗传关系最远。试验结果表明,河南地方野生小鼠与4个近交系小鼠品系之间有遗传相似性,也存在差异,同时也证明RAPD技术可以很好地用于检测小鼠品系间的遗传变异及区分不同的小鼠品系。 |
| 关 键 词： | 野生小鼠 近交系 RAPD 遗传距离 河南省 |
RAPD Analysis of the Wild Mice from Henan and Inbred Strains of Mouse |
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| LEI Jian,HUA Xin-xin,ZHOU Xin,CHAI Bao-guo,ZHAO Wei-dong,ZHENG Zhen-yu.RAPD Analysis of the Wild Mice from Henan and Inbred Strains of Mouse[J].Journal of Henan Agricultural Sciences,2010(5). | |
| Authors: | LEI Jian HUA Xin-xin ZHOU Xin CHAI Bao-guo ZHAO Wei-dong ZHENG Zhen-yu |
| Abstract: | The RAPD analysis of Henan wild mouse and other four inbred strains of mouse were shown in this study.A total of 304 polymorphic fragments were achieved from 380 amplified fragments using 36 polymorphic primers which were selected from 120 random primers.Several specific bands possessed by wild mouse reflected that the RAPD polymorphisms exist among the four inbred strains of mouse and wild mice.The results of statistical analysis of RAPD bands showed that the genetic similarity between wild mice and PWK is 0.00924,which indicated that wild mice had the closest relationship to PWK.However,the genetic similarity between wild mice and DDK is 0.01130,which showed the wild mice were clearly apart from DDK.These results indicated that there were genetic similarities and variations between wild mouse and inbred strains of mouse.Thus,as a molecular marker,RAPD can be used to detect the variations and distinguish the mouse strains. |
| Keywords: | RAPD |
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