Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae) |
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| Authors: | MA Ravaglia FL Lo Nostro MC Maggese GA Guerrero GM Somoza |
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| Institution: | (1) Laboratorio de Embriología Animal, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, 1428 Buenos Aires, Argentina;(2) Facultad de Ciencias Exactas y Naturales, Pabellón II, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
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| Abstract: | This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous
swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine
receptor antagonist, domperidone (DOM) on final maturation and gamete release in this species. Evidence for the presence of
two GnRH variants, sGnRH and cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay
with different antisera. The effects of treatment with sGnRH-A+DOM were checked by three ways: oocyte and milt release by
stripping, histological analysis of the gonadal tissue, and androgen serum levels at different times throughout the experiment.
In males, spermiation was induced after three weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation
at the end of the experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal. All
the treated fish had elevated androgen serum levels from the third week, with respect to control fish. In all cases, serum
estradiol levels were undetectable. These results suggest that treatment with sGnRH analog and DOM induce sex reversal in
female and spermiation in males.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | fish GnRH domperidone hermaphroditism protogynous diandric sex reversal spermiation Synbranchidae |
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