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| 绿僵菌侵染光肩星天牛幼虫过程的透射电镜观察 | |
| 引用本文： | 王达,袁芳芳,黄大庄,刘春延,毕华明.绿僵菌侵染光肩星天牛幼虫过程的透射电镜观察[J].林业科学,2010,46(5). |
| 作者姓名： | 王达 袁芳芳 黄大庄 刘春延 毕华明 |
| 作者单位： | 1. 上海市农业科学院花卉研究中心,上海201106;河北农业大学林学院,保定071000 2. 廊坊市林业局,廊坊,065000 3. 河北农业大学林学院,保定,071000 4. 塞罕坝机械林场,围场县,068400 |
| 基金项目： | 河北农业大学科技将帅计划(Au040201) |
| 摘 要： | 应用透射电镜观察并研究光肩星天牛幼虫感染绿僵菌后体壁和中肠发生的病理变化。结果表明:接菌后16h,寄主表皮层中观察到少量菌丝段,至24h菌丝已普遍侵入,芽管在穿透过程中表现了机械压力和酶活性。36～48h菌丝即可穿透体壁进入血腔。菌丝和菌丝段经血淋巴循环侵入各器官组织。48～72h,随着菌丝和菌丝段在肠壁细胞中迅速大量增殖,肠壁组织逐渐被破坏,中肠微绒毛脱落,肠壁细胞形成空泡,围食膜被分解消失,最终中肠组织崩溃、解体。 |
| 关 键 词： | 绿僵菌 光肩星天牛 侵染过程 病理变化 透射电镜 |
Infection Process of Metarhizium anisopliae in Anoplophora glabripennis Larvae Observed with Transmission Electron Microscopy |
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| Wang Da,Yuan Fangfang,Huang Dazhuang,Liu Chunyan,Bi Huaming.Infection Process of Metarhizium anisopliae in Anoplophora glabripennis Larvae Observed with Transmission Electron Microscopy[J].Scientia Silvae Sinicae,2010,46(5). | |
| Authors: | Wang Da Yuan Fangfang Huang Dazhuang Liu Chunyan Bi Huaming |
| Institution: | 1. Flower Research Center/a>;Shanghai Academy of Agricultural Sciences Shanghai 201106/a>;2. Forestry College/a>;Hebei Agricultural University Baoding 071000/a>;3. Langfang Forestry Administration Langfang 065000/a>;4. Saihanba Machinery Tree Farm Weichang 068400 |
| Abstract: | The pathological changes in integument and midgut of Anoplophora glabripennis larvae infected by Metarhizium anisopliae were studied with a transmission electron microscopy. The results indicated that: in 16 h post-inoculation,the host cuticle was invaded by a few hyphal bodies. Up to 24 h hyphae widely penetrated into host cuticle. The germ tubes showed mechanical pressure and enzymatic activity during the penetration. The hyphae penetrated through the integument and entered the haemocoele from 36 h to 48 ... |
| Keywords: | Metarhizium anisopliae Anoplophora glabripennis infection process pathological changes transmission electron microscopy |
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