| 农杆菌介导小麦花药遗传转化影响因素的研究 |
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| 引用本文： | 赵爱菊,刘玉平,李亚军,程孟恩,李辉,王江浩,陈希勇.农杆菌介导小麦花药遗传转化影响因素的研究[J].河北农业科学,2011,15(2):85-87,91. |
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| 作者姓名： | 赵爱菊 刘玉平 李亚军 程孟恩 李辉 王江浩 陈希勇 |
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| 作者单位： | 1. 河北省农林科学院粮油作物研究所,河北省作物遗传育种实验室,河北石家庄050031
2. 临漳县农业局,河北临漳,056600 |
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| 摘 要： | 采用农杆菌介导法对小麦花药愈伤组织进行转化，并探讨了影响其转化的因素。结果表明：不同基因型的花药培养力存在显著差异，抗性愈伤组织诱导率、抗性芽分化率、抗性植株获得率在不同的基因型之间也存在着很大差异；除小麦品种石4185外，冀5265也是小麦花药农杆菌转化的良好受体基因型；侵染后的愈伤组织在无茵滤纸上共培养2d，效果最好。
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| 关 键 词： | 农杆菌 农杆菌介导法 小麦 花药 愈伤组织 遗传转化 |
Factors Influencing Agrobacterium-mediated Genetic Transformation into Wheat Anther |
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| ZHAO Ai-ju,LIU Yu-ping,LI Ya-jun,CHENG Meng-en,LI Hui,WANG Jiang-hao,CHEN Xi-yong.Factors Influencing Agrobacterium-mediated Genetic Transformation into Wheat Anther[J].Journal of Hebei Agricultural Sciences,2011,15(2):85-87,91. |
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| Authors: | ZHAO Ai-ju LIU Yu-ping LI Ya-jun CHENG Meng-en LI Hui WANG Jiang-hao CHEN Xi-yong |
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| Institution: | 1(1.Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Hebei Crop Genetic Breeding Laboratory,Shijiazhuang 050031,China;2.Agricultural Bureau of Linzhang County,Linzhang 056600,China) |
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| Abstract: | The transformation of wheat anther callus was mediated by Agrobacterium tumefaciens, and the factors influencing transformation were discussed. The results showed that anther culture response of different genotypes had significant difference, the resistant callus induction rate, resistant bud differentiation rate, and resistant plant obtain rate also existed great difference in different genotypes. Ji 5265 was a good receptor genotype for transformation of wheat anther callus as well as Shi 4185. The result was the best, when the infected callus was cultured in sterile filter paper for 2 days. |
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| Keywords: | Agrobacterium tumefaciens Agrobacterium-mediated method Wheat Anther Callus Genetictransformation |
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