| 青贮饲料中乳酸菌的分离鉴定及优良菌株筛选 |
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| 引用本文： | 张慧杰,玉柱,王林,蔡义民,李峰,陶雅,孙启忠.青贮饲料中乳酸菌的分离鉴定及优良菌株筛选[J].草地学报,2011,19(1):137-141. |
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| 作者姓名： | 张慧杰 玉柱 王林 蔡义民 李峰 陶雅 孙启忠 |
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| 作者单位： | 张慧杰,王林,ZHANG Hui-jie,WANG Lin(中国农业科学院研究生院,北京,100081);玉柱,YU Zhu(中国农业大学草地研究所,北京,100094);蔡义民,CAI Yi-min(日本畜产草地研究所,枥木县那须郡,日本,329-2793);李峰,陶雅,孙启忠,LI Feng,TAO Ya,SUN Qi-zhong(中国农业科学院草原研究所,内蒙古,呼和浩特,010010) |
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| 基金项目： | 现代农业产业技术体系建设专项资金，中央级公益性科研院所基本科研业务费专项资金，公益性行业(农业)科研专项经费项目，"948"项目，内蒙古自治区自然科学基金项目 |
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| 摘 要： | 为研究青贮饲料中乳酸菌的分类及形态学与生理生化特性,从苜蓿(Medicago sativa L.)及玉米(Zea mays L.)青贮饲料中分离得到5株乳酸菌.经16S rRNA序列分析,2株(G17,GI24)为乳酸片球菌(Pediococcus acidilactici),其余3株(GI11,GI44,GI62)分...
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| 关 键 词： | 青贮 乳酸菌 优良菌株 |
Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains |
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| ZHANG Hui-jie,YU Zhu,WANG Lin,CAI Yi-min,LI Feng,TAO Ya,SUN Qi-zhong.Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains[J].Acta Agrestia Sinica,2011,19(1):137-141. |
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| Authors: | ZHANG Hui-jie YU Zhu WANG Lin CAI Yi-min LI Feng TAO Ya SUN Qi-zhong |
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| Institution: | 1.Graduate School,Chinese Academy of Agricultural Science,Beijing 100081,China;2.China Agriculture University,Beijing 100094,China;3.National Institute of Livestock and Grassland Science,Nasushiobara 329-2793,Japan;4.Grassland Research Institute,CAAS,Hohhot,Inner Mongolia 010010,China) |
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| Abstract: | To study the taxonomic,morphologic,physiological and biochemical characteristics of lactic acid bacteria(LAB) in silage,five strains of LAB were isolated from alfalfa(Medicago sativa L.)and maize(Zea mays L.)silage.The 16S rRNA sequence analysis indicate that two strains are Pediococcus pentosaceus(GI7,GI24),and the other three are Pediococcus acidilactici,Lactobacillus curvatus and Lactobacillus plantarum(GI11,GI44,GI62).Each is homofermentative lactic acid bacteria and can grow well or weakly at 5,10,40,45℃,and with pH3.0~8.0,3.0% and 6.5% NaCl except that GI44 does not grow at pH3.0,4.0,8.0.Through measuring the rate of lactic acid production and the growth curve,GI62 was selected as eximious strain used for preparing lactic acid bacteria additive due to its rapid acid-production and growth speed. |
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| Keywords: | Silage Lactic acid bacteria Eximious strain |
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