| 一种适用于双向电泳的家蚕微孢子虫总蛋白的制备方法 |
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| 引用本文： | 刘加彬,潘国庆,周泽扬,夏庆友.一种适用于双向电泳的家蚕微孢子虫总蛋白的制备方法[J].蚕学通讯,2002,22(2):8-10. |
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| 作者姓名： | 刘加彬 潘国庆 周泽扬 夏庆友 |
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| 作者单位： | 西南农业大学,农业部蚕桑学重点实验室,重庆北碚,400716 |
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| 摘 要： | 本文对适用于双向电泳的微孢子虫总蛋白提取方法进行了探讨。研究表明，采用液氮多次冻融结合手工研磨，再用裂解液抽提的方法能够得到产率较高且适用于双向电泳的家蚕微孢子虫总蛋白。
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| 关 键 词： | 双向电泳 制备方法 家蚕微孢子虫 总蛋白 提取方法 |
A PREPARATION METHOD FOR TOTAL PROTEIN OF NOSEMA BOMBYCIS SUITABLE FOR 2-D PAGE |
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| Liu Jiabin,Pan Guoqing,Zhou Zeyang,Xia Qingyou.A PREPARATION METHOD FOR TOTAL PROTEIN OF NOSEMA BOMBYCIS SUITABLE FOR 2-D PAGE[J].Newsletter of Sericultural Science,2002,22(2):8-10. |
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| Authors: | Liu Jiabin Pan Guoqing Zhou Zeyang Xia Qingyou |
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| Abstract: | The methods of extraction of total protein of Nosema bombycis (N.b.) for 2D-PAGE (2-direction polyacrylamide gel electrophoresis) were studied and a suitable procedure was identified. Repeated freezing with liquid nitrogen and thawing combined with manual grinding, followed by extraction with cleavage oil, gave a good recovery of total protein of Nosema bombycis suitable for 2D-PAGE. |
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| Keywords: | silkworm Nosema bombycis (N b ) total protein 2D-PAGE |
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