| 相思树的愈伤组织培养及其组织细胞学观察 |
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| 引用本文： | 叶玲娟,赖钟雄,苏齐珍,林姗姗,林庆良.相思树的愈伤组织培养及其组织细胞学观察[J].中国农学通报,2009,25(16):39-44. |
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| 作者姓名： | 叶玲娟 赖钟雄 苏齐珍 林姗姗 林庆良 |
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| 作者单位： | 福建农林大学园艺植物生物工程研究所,福州,350002 |
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| 基金项目： | 福建省科技厅国际合作项目，国家科技支撑计划 |
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| 摘 要： | 本文研究了相思愈伤组织继代培养过程中，激素浓度、光照条件、接种量等各因素对相思愈伤组织培养的影响，研究结果发现，在培养基中添加0.2～0.5mg/L的2，4-D浓度水平最有利于相思愈伤组织的生长。通过不同光照强度的对比试验得出，弱光在保持愈伤组织的形态上较有利。将接种量控制在0.3 g/瓶时，在愈伤组织的增殖和保持能达到最优的效果。同时，通过对不同相思愈伤组织细胞学观察可以看出，不同相思品种的愈伤组织细胞形态各不相同，在培养过程中，愈伤组织的细胞的形态也在变化。
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| 关 键 词： | 节节麦 节节麦 PEG6000 水杨酸 活性氧 抗氧化酶 |
| 收稿时间： | 2008-12-26 |
| 修稿时间： | 2009-03-06 |
The callus culture and cytohistological observation on Acacia. spp |
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| Ye Lingjuan,Lai Zhongxiong,Su Qizhen,Lin Shanshan,Lin Qingliang.The callus culture and cytohistological observation on Acacia. spp[J].Chinese Agricultural Science Bulletin,2009,25(16):39-44. |
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| Authors: | Ye Lingjuan Lai Zhongxiong Su Qizhen Lin Shanshan Lin Qingliang |
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| Institution: | (Institute of Horticultural Biotechnology, Fufian Agriculture and Forestry University, Fuzhou 350002) |
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| Abstract: | The different effects such as plant growth regulators, light conditions, and genotypes on the mainte-nance of the three species of Acacia were compared in the experiment, and the results showed that the medium supplemented with 0.2-1 mg/L 2,4-D was beneficial to the maintenance of callus. Moreover, the callus could be kept loose and soft under the weak light by comparing the effects of different light conditions. And the supe-rior inoculation amount was 0.3 g callus in each bottle. By the histological observation on different callus in acacia, it showed that there were different Morphological cells and callus in different species of Acacia, which also changed during the culture. |
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| Keywords: | Acacia spp callus histological observation |
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