| 猪传染性胃肠炎病毒S基因植物表达载体的构建 |
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| 引用本文： | 王龙涛,葛晨霞,高雯雯,付永平,王丕武,胡桂学.猪传染性胃肠炎病毒S基因植物表达载体的构建[J].吉林农业大学学报,2010,32(6):670-674. |
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| 作者姓名： | 王龙涛 葛晨霞 高雯雯 付永平 王丕武 胡桂学 |
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| 作者单位： | 1. 吉林农业大学动物科学技术学院,长春,130118
2. 吉林农业大学生物技术中心,长春,130118 |
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| 基金项目： | 吉林省科技发展计划项目(20080547) |
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| 摘 要： | 应用RT-PCR技术克隆了猪传染性胃肠炎病毒TGEV-JL株S基因全序列,将其连接到pMD18-T载体。经SacⅠ和BamHⅠ酶切鉴定,其产物全长4320bp。测序后与TH-98等8个TGEV毒株的S基因序列进行比对,同源性为97.6%～99.8%。将该基因插入植物表达载体pBI121的CaMV35S启动子下游,构建高效植物表达载体,转入根癌农杆菌EHA101中。结果表明:成功构建了重组植物表达载体pBI121-S,获得农杆菌工程菌。
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| 关 键 词： | 猪传染性胃肠炎病毒 S基因 植物表达载体 |
| 收稿时间： | 2010-06-10 |
| 修稿时间： | 2010-08-07 |
Construction of Plant Expression Vector of Porcine Transmissible Gastroenteritis Virus'S Gene |
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| WANG Long-tao,GE Chen-xia,GAO Wen-wen,FU Yong-ping,WANG Pi-wu,HU Gui-xue.Construction of Plant Expression Vector of Porcine Transmissible Gastroenteritis Virus'S Gene[J].Journal of Jilin Agricultural University,2010,32(6):670-674. |
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| Authors: | WANG Long-tao GE Chen-xia GAO Wen-wen FU Yong-ping WANG Pi-wu HU Gui-xue |
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| Institution: | 1.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2.Biotechnology Center of Jilin Agricultural University,Changchun 130118, China |
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| Abstract: | The full length DNA fragment of S gene of porcine transmissible gastroenteritis virus was amplified from the strain of TGEV JL by RT-PCR technique.Then, the fragment was connected to the pMD18 T vector. The SacⅠ and BamHⅠ restriction enzyme digestion analysis showed that the length of the product was 4 320 bp. After sequencing, S gene sequence of TH-98, etc. 8 TGEV strains were compared, and homology was 97.6％—993.8％. The gene was inserted into plant expression vector pBI121 to construct efficient plant expression vector at downstream of the CaMV35S promoter, inserted into Agrobacterium tumefaciens EHA101. The results showed that the recombinant plant expression vector pBI121-S and Agrobacterium tumefaciens strain were constructed. |
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| Keywords: | plant expression vector |
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