| 根结线虫种群的线粒体DNA分析 |
| |
| 引用本文： | 孙龙华,廖金铃,李迅东,卓侃.根结线虫种群的线粒体DNA分析[J].植物病理学报,2005,35(2):134-140. |
| |
| 作者姓名： | 孙龙华 廖金铃 李迅东 卓侃 |
| |
| 作者单位： | 华南农业大学资源环境学院, 广州 510642 |
| |
| 基金项目： | 中国科学院资助项目，国家科技攻关项目 |
| |
| 摘 要： | 在同工酶和形态学鉴定的基础上,利用引物#C2F3和#1108对42个根结线虫种群线粒体DNA (mtDNA)中的COⅡ和LrRNA间区域进行特异性PCR扩增,35个种群的扩增产物约为1.7kb,其中29个是南方根结线虫,6个是爪哇根结线虫;3个花生根结线虫种群的扩增产物约为1.1kb;1个种群的扩增产物约为0.7kb,为根结线虫属在中国的新记录种;3个北方根结线虫种群的扩增产物约为0.5kb。用单条2龄幼虫提取物作模板得到的结果与大量提取DNA作模板的结果相同。为了区分产生相同大小片段的南方根结线虫和爪哇根结线虫,用限制性内切酶HinfⅠ对扩增产物进行酶切,结果表明:所有供试的南方根结线虫都可以被HinfⅠ酶切,且产生约1.3和0.4kb的2个限制性片段;但供试的爪哇根结线虫种群不能被酶切。由此表明,利用mtDNA PCR及酶切实验可以作为快速而准确地鉴定常见根结线虫的方法。
|
| 关 键 词： | 根结线虫 mtDNA 鉴定 PCRRFLP 中国 |
| 文章编号： | 0412-0914(2005)02-0134-07 |
| 修稿时间： | 2004年7月15日 |
Analysis of root-knot nematode species and populations based on mitochondrial DNA |
| |
| SUN Long-hua,LIAO Jin-ling,LI Xun-dong,ZHUO Kan.Analysis of root-knot nematode species and populations based on mitochondrial DNA[J].Acta Phytopathologica Sinica,2005,35(2):134-140. |
| |
| Authors: | SUN Long-hua LIAO Jin-ling LI Xun-dong ZHUO Kan |
| |
| Institution: | College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China |
| |
| Abstract: | Meloidogyne incognita, M. javanica and M. arenaria have their representative isozyme phenotypes. Primers #C2F3 and #1108 were utilized to amplify the intergenic region between COⅡ and LrRNA genes of mtDNA of 42 Meloidogyne populations. Specific amplified fragments were about 1.7 kb for 35 Meloidogyne populations, including 29 M. incognita populations and 6 M. javanica populations; about 1.1 kb for 3 M. arenaria populations; about 0.7 kb for 1 population of a new record root-knot nematode species; and about 0.5 kb for 3 M. hapla populations. The PCR results gained from DNA of the single juvenile and the mass juveniles were the same. The amplified products were digested with the restriction enzyme HinfⅠ, and the results showed that all populations of M. incognita can be digested into two restriction fragments of about 1.3 and 0.4 kb, but the PCR product of specific region on mtDNA of M. javanica can not be digested. It concluded that mtDNA was a rapid and reliable approach for molecular identification of common Meloidogyne species. |
| |
| Keywords: | mtDNA PCR-RFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
