Comparing p20’s RNA silencing suppressing activity among five phylogenetic groups of <Emphasis Type="Italic">Citrus Tristeza virus</Emphasis> |
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| Authors: | Natália T Marques Ângela A Costa Débora Lopes Gonçalo Silva Gustavo Nolasco |
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| Institution: | 1.Center for Biodiversity, Functional and Integrative Genomics,Universidade do Algarve,Faro,Portugal;2.Faculdade de Ciências e Tecnologia,Universidade do Algarve,Faro,Portugal |
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| Abstract: | The p20 protein encoded by the Citrus Tristeza Virus (CTV) was previously identified as a RNA silencing suppressor. In this study, we analyzed the p20’s suppressing activity
from five phylogenetic groups of CTV, using the co-infiltration assay of Green fluorescence protein (GFP) gene and the suppressor
gene in 16C line Nicotiana benthamiana plants. Green fluorescence, GFP mRNA relative levels and GFP specific siRNAS were compared showing in most cases, only slight
differences. Contrary to previous studies, the p20 suppressor was not able to impede neither short range nor systemic spreading
of RNA silencing. The suppressor from the phylogenetic group 4 revealed a much reduced activity when compared with the others.
At present we still don’t know whether this property is a characteristic of this group or an atypical feature due to a unique
point mutation. The differences in the symptom type and intensity originated by isolates belonging to the phylogenetic groups
assayed could not be related to differences to the p20 suppressor’s activity. |
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