| 保护剂和再水化剂对冷冻干燥保存的生防菌YT11的存活率及生防效果的影响 |
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| 引用本文： | 詹袆,王云鹏,郭坚华.保护剂和再水化剂对冷冻干燥保存的生防菌YT11的存活率及生防效果的影响[J].中国生物防治,2011,27(1):110-114. |
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| 作者姓名： | 詹袆 王云鹏 郭坚华 |
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| 作者单位： | 南京农业大学植物保护学院植物病理学系/江苏省生物源农药工程中心/农业部作物病虫害监测与防控重点开放实验室,南京,210095 |
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| 基金项目： | 农业部行业(农业)科研专项(200903052); 江苏省科技创新与成果转化专项引导资金项目(BY2009157) |
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| 摘 要： | 本文研究了多种保护剂和再水化剂对生防菌成团泛菌Pantoea ananatis菌株YT11在冷冻干燥保存中存活率的影响,比较了不同保存温度对YT11贮藏稳定性和生防活力的差异.结果表明,5%蔗糖是最佳的保护剂,冷冻干燥后菌株YT11存活率达90%以上.以5%蔗糖作保护剂时,5%脱脂奶粉为最佳再水化剂,菌株YT11复苏率达100%.贮藏试验结果表明,4℃贮存的冷冻干燥粉剂的菌株存活率显著高于22℃下的存活率.对保存24周后的菌剂进行温室生防试验,结果显示,4℃和22℃下保存的菌剂对番茄青枯病的防效分别达到71.57%和50.98%.因此,以5%蔗糖为保护剂和5%脱脂奶粉为再水化剂,对成团泛菌菌株YT11进行冷冻干燥处理,再将冷冻干燥后的菌株于4℃贮存,可有效保证生防菌的生防活力.
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| 关 键 词： | 冷冻干燥保护剂 再水化剂 成团泛菌 存活率 |
Impact of Protective Agents and Rehydration Media on Viability and Efficacy of the Freeze-dried Biocontrol Agent Pantoea ananatis Strain YT11 |
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| ZHAN Yi
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WANG Yun-peng
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GUO Jian-hua.Impact of Protective Agents and Rehydration Media on Viability and Efficacy of the Freeze-dried Biocontrol Agent Pantoea ananatis Strain YT11[J].Chinese Journal of Biological Control,2011,27(1):110-114. |
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| Authors: | ZHAN Yi WANG Yun-peng GUO Jian-hua |
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| Institution: | ZHAN Yi,WANG Yun-peng,GUO Jian-hua(Department of Plant Pathology,College of Plant Protection,Engineering Center of Bioresource Pesticide in Jiangsu Province,KeyLaboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing 210095,China) |
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| Abstract: | The effect of different protective agents and rehydration media on the viabilities of the biocontrol agent Pantoea ananatis strain YT11 subjected to freeze-drying,its subsequent survival rates during storage under different temperatures,and the biocontrol efficacies of the freeze-dried cells were investigated.Results indicated that 5% sucrose gave the greatest protection to YT11 with cell viability more than 90% during freeze-drying,and 5% non-fat skim milk as rehydration media gave the highest survival rat... |
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| Keywords: | protective agents rehydration medium Pantoea ananatis viability |
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