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| 不同CSF免疫状态下猪PRRS易感性及IFN-γ分泌细胞应答 | |
| 引用本文： | 白雪,付云云,单月,郭敏,郎广平,梅林,温涛,赵建增,梁志选,杨举,罗胜军,邢海云,高英杰.不同CSF免疫状态下猪PRRS易感性及IFN-γ分泌细胞应答[J].中国兽医学报,2012,32(1):5-7,12. |
| 作者姓名： | 白雪 付云云 单月 郭敏 郎广平 梅林 温涛 赵建增 梁志选 杨举 罗胜军 邢海云 高英杰 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林长春,130062 2. 吉林大学畜牧兽医学院,吉林长春130062／华威特（北京）生物科技有限公司,北京100085 3. 天津市动物疫病预防控制中心,天津,300402 4. 广东省农业科学院兽医研究所,广东广州,510640 |
| 基金项目： | 国家自然科学基金资助项目(30972193) |
| 摘 要： | 采用酶联免疫斑点检测技术（ELISpot）检测自然状态下猪外周血单核细胞（PBMC）中分泌IFN-γ的细胞数，并用带T细胞表位的猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus，PRRSV）特异性小分子多肽刺激培养的PBMC，观察IFN-γ的分泌变化。结果显示，猪瘟病毒（Classical fever virus，CSFV）抗体阳性组中感染PRRSV比率小于CSFV抗体阴性组。CSFV抗体阳性猪PBMC中IFN-γ分泌细胞数量均高于CS—FV抗体阴性组，CSFV抗体阴性且受PRRSV感染猪的PBMC对PRRSV多肽刺激不应答。结果表明，对CSFV疫苗应答好的猪对PRRSV感染有一定的抵抗，其细胞免疫处于活动状态，提示2种传染病的免疫应答机理有部分相关性。 |
| 关 键 词： | 猪瘟疫苗 猪繁殖与呼吸综合征病毒 酶联免疫斑点检测技术 IFN-γ |
Susceptibility to porcine reproductive and respiratory syndrome(PRRS) and response to IFN-γ secreting cells in swine vaccinated with classical swine virus |
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| BAI Xue,FU Yun-yun,SHAN Yue,GUO Min,LANG Guang-ping,MEI Lin,WEN Tao,ZHAO Jian-zeng,LIANG Zhi-xuan,YANG Ju,LUO Sheng-jun,XING Hai-yun,GAO Ying-jie.Susceptibility to porcine reproductive and respiratory syndrome(PRRS) and response to IFN-γ secreting cells in swine vaccinated with classical swine virus[J].Chinese Journal of Veterinary Science,2012,32(1):5-7,12. | |
| Authors: | BAI Xue FU Yun-yun SHAN Yue GUO Min LANG Guang-ping MEI Lin WEN Tao ZHAO Jian-zeng LIANG Zhi-xuan YANG Ju LUO Sheng-jun XING Hai-yun GAO Ying-jie |
| Institution: | 1*(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Beijing Sinovet Biotech Co.Ltd,Beijing 100085,China;3.Tianjin Animal Diseases Control Center,Tianjin 300402,China;4.Institute of Veterinary Medicine,GDAAS,Guangzhou 510640,China) |
| Abstract: | We use the ELISpot to monitor the IFN-γsecreting cells in peripheral blood and detect the dynamic changes of secretion IFN-γ after treated with PRRSV-specific peptide carrying T cell epitopes.The high-CSFV antibody groups have a lower PRRSV infection rate than the low-CSFV antibody groups;the high-CSFV antibody groups display a higher number of IFN-γ secreting cells.The PBMC of porcine affected by PRRSV infection in the low-CSFV antibody groups did not respond to stimulus with PRRSV-specific peptides.Pigs can be protected from PRRSV which had good response to CSFV vaccine,and their cellular immunity is active,which suggest that there is a relevance between immune response mechanism of CSF and PRRS to some extent. |
| Keywords: | CSFV vaccine PRRSV ELISpot IFN-γ |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |