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| NDV对雏鸵鸟HPA轴的影响 | |
| 引用本文： | 唐丽,位兰,彭克美.NDV对雏鸵鸟HPA轴的影响[J].中国兽医学报,2012,32(3):337-340. |
| 作者姓名： | 唐丽 位兰 彭克美 |
| 作者单位： | 1. 四川农业大学动物医学院,四川雅安625014／华中农业大学动物医学院,湖北武汉430070 2. 河南科技大学动物科技学院,河南洛阳,471003 3. 华中农业大学动物医学院,湖北武汉,430070 |
| 基金项目： | 国家自然科学基金资助项目(39970547,30471249);博士专项基金资助项目(00270403) |
| 摘 要： | 以45日龄雏鸵鸟为试验动物,分正常组和攻毒组,利用RIA技术和TUNEL技术,探讨新城疫病毒（NDV）对雏鸵鸟HPA轴的影响。结果表明,雏鸵鸟感染NDV,HPA轴内细胞凋亡数量在病毒感染期间明显高于对照组,提示NDV能诱导雏鸵鸟HPA轴系统发生细胞凋亡;在病毒接种后1d,肾上腺内可检测到大量凋亡细胞,接种5d后凋亡数量显著增加（P〈0.05）,之后凋亡呈下降趋势,肾上腺内细胞凋亡的动态变化反映了雏鸵鸟HPA轴对ND病变的适应和调节。雏鸵鸟感染NDV,血清ACTH水平于NDV接种后1d开始上升,至5d达到峰值（P〈0.01）,之后有所下降,渐趋于正常;血清Cor水平于病毒接种后1d开始下降,至7d有所回升,9d渐趋于正常水平,表明NDV接种后雏鸵鸟血清ACTH和Cor水平的变化与其HPA轴功能的损伤及恢复密切相关。 |
| 关 键 词： | 雏鸵鸟 下丘脑-垂体-肾上腺轴 新城疫 细胞凋亡 激素 |
Effect of NDV on HPA axis in ostrich chicks |
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| TANG Li,WEI Lan,PENG Ke-mei.Effect of NDV on HPA axis in ostrich chicks[J].Chinese Journal of Veterinary Science,2012,32(3):337-340. | |
| Authors: | TANG Li WEI Lan PENG Ke-mei |
| Institution: | 1.College of Veterinary Medicine,Sichuan Agricultural University,Ya′an,Sichuan 625014,China;2.College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;3.College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,Henan 471003,China) |
| Abstract: | In the present study,ostrich chicks aged 45 days were divided into normal group and challenge group at random.The methods of RIA and TUNEL were used to study the effect of Newcastle disease virus(NDV) on HPA axis in ostrich chicks.The results were as follows:after artificially infected with NDV,the apoptosis in HPA axis of challenge group increased obviously,indicating that NDV can induce the apoptosis of HPA axis of ostrich chicks.In adrenal gland the number of apoptosis increased significantly at 1 day after challenged,and reached maximum at 5 days(P<0.01),then delined.The dynamic changes of apoptosis in adrenal gland indicated the adapting and regulating of HPA axis in the pathological stress reaction.The level of ACTH in serum increased slightly at 1 day after challenged,reached its maximum at 5 day(P<0.01),then came back.The level of serum cortisol increased at 7 day after challenged,then gradually became normal at 9 day.These results demonstrate that the variation of ACTH and cortisol in serum of challenge group correlates closely with the damage and restoration process of HPA axis. |
| Keywords: | ostrich chicks hypothalamus-pituitary-adrenal axis Newcastle disease apoptosis hormone |
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