Chromosomal regions associated with barley yellow dwarf virus resistance in oat |
| |
| Authors: | José F Barbosa-Neto Wilawan Siripoonwiwat Louise S O'Donoughue Stewart M Gray Down M Smith Fred L Kolb Catherine Gourmet Charlie M Brown Mark E Sorrells |
| |
| Institution: | (1) John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, U.K. |
| |
| Abstract: | Immature embryos of the spring barley variety GoldenPromise, were bombarded with three different particledelivery systems and both transient and stabletransformation examined. In addition, a range oftechniques for the preparation of the DNA coated goldparticles was examined. Fertile transgenic barleyplants were obtained using three particle preparationtechniques which differed in the amount of gold andDNA used for each bombardment. However, only one ofthe particle delivery systems, the PDS 1000/He device,appeared to be effective in yielding transformedbarley plants. |
| |
| Keywords: | Barley Hordeum vulgare transformation particle bombardment particle gun |
| 本文献已被 SpringerLink 等数据库收录！ |
|
