| 发芽蚕豆β-淀粉酶的提取研究 |
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| 引用本文： | 王慧超,冉燕,乔辰.发芽蚕豆β-淀粉酶的提取研究[J].安徽农业科学,2010,38(1):345-346. |
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| 作者姓名： | 王慧超 冉燕 乔辰 |
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| 作者单位： | 长江师范学院生命科学与技术学院,重庆,408100 |
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| 摘 要： | 目的]优化发芽蚕豆β-淀粉酶提取条件。方法]采用3，5-二硝基水杨酸法。结果]选用1．0g／L Na2SO3和1．5g／LNaHSO3为还原剂时，发芽蚕豆β-淀粉酶提取效果较好；选用pH值5．5柠檬酸缓冲液和pH值6．5磷酸盐缓冲液时，发芽蚕豆β-淀粉酶提取效果较好。结论]以1．0g/L Na2SO4为还原剂，以pH值6．5磷酸钾盐缓冲液为提取缓冲液，提取发芽蚕豆β-淀粉酶效果较好。
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| 关 键 词： | 蚕豆 β-淀粉酶 提取 |
Study on Extracting β-Amylase from Germinated Broad Bean |
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| WANG Hui-chao et al.Study on Extracting β-Amylase from Germinated Broad Bean[J].Journal of Anhui Agricultural Sciences,2010,38(1):345-346. |
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| Authors: | WANG Hui-chao |
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| Institution: | College of Life Science and Technology;Yangtze Normal University;Chongqing 408100 |
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| Abstract: | Objective]The extraction condition was optimized. Method] 3,5-dinitrosalicylic acid regent was used in this study. Result] The extraction effect of β-amylase from the germinated broad bean was better with 1.0 g/L Na_2SO_3 and 1.5 g/L NaHSO_3 as reducing agent. The extraction effect of β-amylase from the germinated broad bean was better with pH 5.5 citric acid buffer solution and pH 6.5 phosphate buffer solution. Conclusion] The extraction effect of β-amylase from the germinated broad bean was better with 1.0 g/L Na_2SO_3 as reducing agent and pH 6.5 tribasic potassium phosphate buffer solution as extraction buffer solution. |
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| Keywords: | Broad bean Extraction |
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