| 用反相高效液相色谱-紫外检测法测定桑叶中芦丁、槲皮素和1-脱氧野尻霉素的含量 |
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| 引用本文： | 吴秋生,王俊,吴福安,吴倩,刘利,陈明胜,赵璐璐,程嘉翎.用反相高效液相色谱-紫外检测法测定桑叶中芦丁、槲皮素和1-脱氧野尻霉素的含量[J].蚕业科学,2009,35(1). |
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| 作者姓名： | 吴秋生 王俊 吴福安 吴倩 刘利 陈明胜 赵璐璐 程嘉翎 |
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| 作者单位： | 1. 江苏科技大学生物与环境工程学院,江苏,镇江,212018
2. 中国农业科学院蚕业研究所,江苏,镇江,212018 |
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| 基金项目： | 江苏省高等学校大学生实践创新训练计划项目，江苏省高校自然科学基础研究面上项目 |
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| 摘 要： | 为高效检测桑叶中的药用活性成分,进行了用反相高效液相色谱-紫外检测法(RP-HPLC-UV)综合测定桑叶中芦丁、槲皮素和1-脱氧野尻霉素(1-DNJ)含量的试验。采用85%乙醇超声辅助萃取桑叶中的芦丁和槲皮素,以0.05 mol/L HCl为溶剂提取1-DNJ,并在pH8.5的硼酸盐缓冲液中进行衍生化反应,生成具有紫外吸收的1-DNJ-FMOC络合物。色谱柱为Alltima C18(4.6 mm×250 mm,5μm),检测波长分别为360、360、254 nm,流动相分别为V(四氢呋喃)∶V(0.02%磷酸)=25∶75、V(乙腈)∶V(pH4.0、0.5 mol/L醋酸缓冲液)=27∶73、V(乙腈)∶V(0.1%醋酸)=36∶64。检测结果显示:芦丁、槲皮素和1-DNJ的色谱图分离效果良好,峰面积与浓度呈高度正相关,回收率(n=3)分别为99.14%、98.89%、100.85%,相对标准偏差(RSD)分别为1.01%、0.33%、1.24%。试验显示建立在以上条件下的检测方法精密度高,检测结果准确可靠。
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| 关 键 词： | 桑叶 芦丁 槲皮素 1-脱氧野尻霉素 反相高效液相色谱-紫外检测法 |
Determination of Rutin,Quercertin and 1-Deoxynojirimycin in Mulberry Leaves by RP-HPLC-UV |
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| WU Qiu-Sheng,WANG Jun,WU Fu-An,WU Qian,LIU Li,CHEN Ming-Sheng,ZHAO Lu-Lu,CHENG Jia-Ling.Determination of Rutin,Quercertin and 1-Deoxynojirimycin in Mulberry Leaves by RP-HPLC-UV[J].Acta Sericologica Sinica,2009,35(1). |
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| Authors: | WU Qiu-Sheng WANG Jun WU Fu-An WU Qian LIU Li CHEN Ming-Sheng ZHAO Lu-Lu CHENG Jia-Ling |
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| Institution: | WU Qiu-Sheng1 WANG Jun1 WU Fu-An2 WU Qian1 LIU Li2CHEN Ming-Sheng2 ZHAO Lu-Lu1 CHENG Jia-Ling2(1College of Biological , Environmental Engineering,Jiangsu University of Science , Technology,Zhenjiang Jiangsu 212018,China,2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,China) |
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| Abstract: | Rutin,quercertin and 1-DNJ(1-deoxynojirimycin) are active medicinal constituents in mulberry leaves.In order to determine these constituents by RP-HPLC(reversed-phase high performance liquid chromatography),rutin and quercertin were extracted with 85% ethanol by ultrasonic-assisted extraction technology and 1-DNJ was extracted with 0.05 mol/L HCl,and derivatized by FMOC-Cl in pH 8.5 borate buffer solution to yield DNJ-FMOC,which can be detected with ultraviolet.Rutin,quercertin and 1-DNJ were separated by A... |
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| Keywords: | Mulberry leaf Rutin Quercertin 1-deoxynojirimycin RP-HPLC-UV |
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